Centre in Regenerative Medicine, Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, Queensland, Australia.
Department of Plastic & Reconswrapping a sterile Coban wrap around the limb distallytructive Surgery, Princess Alexandra Hospital, Woolloongabba, Queensland, Australia.
Nat Protoc. 2020 Mar;15(3):877-924. doi: 10.1038/s41596-019-0271-2. Epub 2020 Feb 14.
Critical-size bone defects, which require large-volume tissue reconstruction, remain a clinical challenge. Bone engineering has the potential to provide new treatment concepts, yet clinical translation requires anatomically and physiologically relevant preclinical models. The ovine critical-size long-bone defect model has been validated in numerous studies as a preclinical tool for evaluating both conventional and novel bone-engineering concepts. With sufficient training and experience in large-animal studies, it is a technically feasible procedure with a high level of reproducibility when appropriate preoperative and postoperative management protocols are followed. The model can be established by following a procedure that includes the following stages: (i) preoperative planning and preparation, (ii) the surgical approach, (iii) postoperative management, and (iv) postmortem analysis. Using this model, full results for peer-reviewed publication can be attained within 2 years. In this protocol, we comprehensively describe how to establish proficiency using the preclinical model for the evaluation of a range of bone defect reconstruction options.
临界尺寸骨缺损需要大量的组织重建，仍然是临床面临的挑战。骨工程有潜力提供新的治疗概念，但临床转化需要解剖学和生理学相关的临床前模型。绵羊临界尺寸长骨缺损模型已在许多研究中得到验证，可作为评估传统和新型骨工程概念的临床前工具。在具有丰富的大动物研究经验和训练的情况下，当遵循适当的术前和术后管理方案时，该模型是一种技术上可行且具有高度可重复性的程序。该模型可以通过以下步骤建立：（i）术前规划和准备，（ii）手术入路，（iii）术后管理，和（iv）死后分析。使用该模型，在 2 年内可以获得完整的同行评审出版物结果。在本方案中，我们全面描述了如何使用临床前模型来评估一系列骨缺损重建方案，从而建立熟练程度。
