Edosuyi Osaze, Igbe Ighodaro, Iniaghe Loretta Oghenekome
Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Benin, Benin City, Nigeria.
J Complement Integr Med. 2017 Dec 5;15(1):/j/jcim.2018.15.issue-1/jcim-2017-0099/jcim-2017-0099.xml. doi: 10.1515/jcim-2017-0099.
Background Hunteria umbellata (HU) (K. Schum) is used in ethnomedicine for the management of pain, diabetes mellitus and dysmenorrhoea. This study evaluated the analgesic and antioxidant activities of aqueous extract of HU stem bark and the possible mechanism(s) of action. Methods The antinociceptive effect of HU was evaluated using acetic acid mouse writhing, tail flick, hot plate and formalin-induced paw licking models. To establish the possible mechanism(s) of action of HU, separate group of animals were pretreated with naloxone (1 mg/kg, i.p.), atropine (1 mg/kg, i.p.), haloperidol (0.1 mg/kg, i.p.), ondansetron (1 mg/kg, i.p.) and phenoxybenzamine (0.1 mg/kg, i.p.), 15 min before HU. The in vivo and in vitro antioxidant potential was evaluated using established methods. Results The extract at 150 and 300 mg/kg, significantly (p<0.05) reduced the number of writhes and paw licking times and increased pain threshold in writhing assay, paw licking and hotplate tests respectively. Pretreatment of animals with ondansetron, naloxone and haloperidol, significantly (p<0.05 and p<0.01) attenuated the analgesic activity of HU. The extract demonstrated significant (p<0.05) radical scavenging activity (IC50 0.39 µg/mL), with high phenol content and reducing property. The total phenol content was 124.19 per gram of gallic acid. In vivo antioxidant assay showed significant (p<0.05) increase in catalase and superoxide levels. Conclusions Results obtained in this study suggest the involvement of serotonergic, opioidergic and dopaminergic pathways in the analgesic effect of HU stem bark, in addition to its potent antioxidant potential.
伞花萝芙木（Hunteria umbellata，简称HU）（K. Schum）在民族医学中用于治疗疼痛、糖尿病和痛经。本研究评估了HU茎皮水提取物的镇痛和抗氧化活性及其可能的作用机制。方法：采用醋酸致小鼠扭体、甩尾、热板和福尔马林致爪舔模型评估HU的抗伤害感受作用。为确定HU可能的作用机制，在给予HU前15分钟，分别用纳洛酮（1 mg/kg，腹腔注射）、阿托品（1 mg/kg，腹腔注射）、氟哌啶醇（0.1 mg/kg，腹腔注射）、昂丹司琼（1 mg/kg，腹腔注射）和酚苄明（0.1 mg/kg，腹腔注射）预处理动物组。采用既定方法评估体内和体外抗氧化潜力。结果：提取物在150和300 mg/kg时，分别显著（p<0.05）减少了扭体次数和爪舔次数，并分别提高了扭体试验、爪舔试验和热板试验中的疼痛阈值。用昂丹司琼、纳洛酮和氟哌啶醇预处理动物，显著（p<0.05和p<0.01）减弱了HU的镇痛活性。提取物表现出显著（p<0.05）的自由基清除活性（IC50为0.39 µg/mL），具有高酚含量和还原特性。总酚含量为每克没食子酸124.19。体内抗氧化试验显示过氧化氢酶和超氧化物水平显著（p<0.05）升高。结论：本研究结果表明，除了具有强大的抗氧化潜力外，HU茎皮的镇痛作用还涉及5-羟色胺能、阿片样物质能和多巴胺能途径。
