Department of Pharmacy, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka, 1217, Bangladesh.
Department of Pharmacy, State University of Bangladesh, Dhaka, 1205, Bangladesh.
BMC Complement Altern Med. 2019 Aug 14;19(1):214. doi: 10.1186/s12906-019-2565-x.
The present study evaluated the antinociceptive effect of the bark of Artocarpus lacucha, which is used for the treatment of stomachache, headache and boils in the traditional system of medicine.
The antinociceptive activity was investigated by the tail immersion, hot plate, acetic acid- & formalin-induced nociception and carrageenan-induced paw edema tests using a hydro-methanolic extract of A. lacucha bark. The plant extract was found to contain a substantial amount of phenolic compounds according to the total phenolic and flavonoid content assay. A phenolic metabolite, (+)-catechin, has been isolated using different chromatographic techniques. The compound was characterized with 1D and 2D NMR spectroscopic data. (+)-catechin, isolated from A. lacucha was assessed for antinociceptive effects swiss albino mice. Furthermore, the possible involvement of opioid receptors and ATP-sensitive K channel for the effect of the plant extract and (+)-catechin has been justified using naloxone and glibenclamide, respectively.
Oral administration (p.o) of the plant extract (50-200 mg/Kg b.w.) resulted in significant thermal pain protection in the hot plate and tail immersion tests. The action of the plant extract was significantly antagonized by naloxone, a non-selective opioid antagonist, in the hot plate and tail immersion tests, which supports the involvement of opioid receptors. Both the plant extract and (+)-catechin, (50-200 mg/Kg b.w., p.o.) significantly diminished the acetic acid- & formalin-induced nociception, and carrageenan-induced paw edema. Glibenclamide, an ATP-sensitive K channel blocker, significantly reversed their effect in the acetic acid-induced writhing test which indicates the participation of ATP-sensitive K channel system.
The investigation revealed potential central and peripheral antinociceptive effects of A. lacucha bark supports its applications in the traditional system of medicine.
本研究评估了 Artocarpus lacucha 树皮的镇痛作用，该树皮在传统医学中用于治疗胃痛、头痛和痈。
采用尾浸法、热板法、醋酸-甲醛诱导的疼痛和角叉菜胶诱导的爪肿胀试验，研究了 Artocarpus lacucha 树皮的水-甲醇提取物的镇痛活性。根据总酚和类黄酮含量测定，发现该植物提取物含有大量酚类化合物。采用不同的色谱技术分离出一种酚类代谢物（+）-儿茶素。该化合物通过 1D 和 2D NMR 光谱数据进行了表征。（+）-儿茶素是从 Artocarpus lacucha 中分离出来的，用于评估其对瑞士白化小鼠的镇痛作用。此外，分别使用纳洛酮和格列本脲，证明了植物提取物和（+）-儿茶素的作用可能涉及阿片受体和 ATP 敏感性钾通道。
口服（p.o）植物提取物（50-200mg/Kgb.w.）可显著减轻热板和尾浸试验中的热痛。纳洛酮，一种非选择性阿片受体拮抗剂，显著拮抗了植物提取物在热板和尾浸试验中的作用，这支持了阿片受体的参与。植物提取物和（+）-儿茶素（50-200mg/Kgb.w.，p.o.）均显著减轻了醋酸-甲醛诱导的疼痛和角叉菜胶诱导的爪肿胀。ATP 敏感性钾通道阻滞剂格列本脲显著逆转了它们在醋酸诱导的扭体试验中的作用，这表明 ATP 敏感性钾通道系统的参与。
研究结果表明，Artocarpus lacucha 树皮具有潜在的中枢和外周镇痛作用，支持其在传统医学中的应用。
