• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

miR-328-3p 通过 H2AX 增强骨肉瘤的放射敏感性并调节细胞凋亡和细胞活力。

miR‑328‑3p enhances the radiosensitivity of osteosarcoma and regulates apoptosis and cell viability via H2AX.

机构信息

Guizhou Provincial People's Hospital, Guiyang, Guizhou, P.R. China.

Affiliated Hospital of Zunyi Medical College, Zunyi, Guizhou, P.R. China.

出版信息

Oncol Rep. 2018 Feb;39(2):545-553. doi: 10.3892/or.2017.6112. Epub 2017 Nov 27.

DOI:10.3892/or.2017.6112
PMID:29207178
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5783622/
Abstract

Osteosarcoma is a kind of high-risk sarcoma of the skeleton typically observed in people under 25 years old. Currently, radiotherapy is widely applied in cancer treatment. However, osteosarcoma is radioresistant and accordingly new, more effective radiosensitizers are needed. miRNAs have been reported to play an important role in osteosarcoma radiosensitivity. We examined the modulating effect of miR‑328‑3p in vivo and in vitro. miR‑328‑3p was downregulated in HOS‑2R cells. The overexpression of miR‑328‑3p enhanced the radiosensitivity of osteosarcoma cells. miR‑328‑3p inhibited proliferation and promoted apoptosis in osteosarcoma cells under radiation conditions. In cells overexpressing miR‑328‑3p, H2AX expression was downregulated. We found that miR‑328‑3p targets H2AX and inhibits its expression. It was concluded, that miR‑328‑3p enhances the radiosensitization of osteosarcoma following X-ray irradiation, and determined that it directly targets H2AX to regulate radiosensitization.

摘要

骨肉瘤是一种高发于 25 岁以下人群的骨骼肉瘤。目前，放射疗法被广泛应用于癌症治疗。然而，骨肉瘤具有放射抵抗性，因此需要新的、更有效的放射增敏剂。有研究报道，miRNA 在骨肉瘤放射敏感性中发挥着重要作用。我们在体内和体外研究了 miR-328-3p 的调节作用。miR-328-3p 在 HOS-2R 细胞中呈下调表达。过表达 miR-328-3p 可增强骨肉瘤细胞的放射敏感性。miR-328-3p 可抑制辐射条件下骨肉瘤细胞的增殖并促进其凋亡。在过表达 miR-328-3p 的细胞中，H2AX 表达下调。我们发现 miR-328-3p 可靶向 H2AX 并抑制其表达。因此，我们得出结论，miR-328-3p 可增强骨肉瘤对 X 射线照射的放射敏感性，并确定其可通过直接靶向 H2AX 来调节放射敏感性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73f8/5783622/5c34bbd58ff8/OR-39-02-0545-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73f8/5783622/3f9828203fa0/OR-39-02-0545-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73f8/5783622/b2e88b3da12f/OR-39-02-0545-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73f8/5783622/4c70905de10d/OR-39-02-0545-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73f8/5783622/19a425db30da/OR-39-02-0545-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73f8/5783622/fb60b8f0aaa3/OR-39-02-0545-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73f8/5783622/5c34bbd58ff8/OR-39-02-0545-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73f8/5783622/3f9828203fa0/OR-39-02-0545-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73f8/5783622/b2e88b3da12f/OR-39-02-0545-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73f8/5783622/4c70905de10d/OR-39-02-0545-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73f8/5783622/19a425db30da/OR-39-02-0545-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73f8/5783622/fb60b8f0aaa3/OR-39-02-0545-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73f8/5783622/5c34bbd58ff8/OR-39-02-0545-g05.jpg

相似文献

1
miR‑328‑3p enhances the radiosensitivity of osteosarcoma and regulates apoptosis and cell viability via H2AX.miR-328-3p 通过 H2AX 增强骨肉瘤的放射敏感性并调节细胞凋亡和细胞活力。
Oncol Rep. 2018 Feb;39(2):545-553. doi: 10.3892/or.2017.6112. Epub 2017 Nov 27.
2
MiR-564 is down-regulated in osteosarcoma and inhibits the proliferation of osteosarcoma cells via targeting Akt.微小RNA-564在骨肉瘤中表达下调，并通过靶向Akt抑制骨肉瘤细胞的增殖。
Gene. 2018 Mar 1;645:163-169. doi: 10.1016/j.gene.2017.12.028. Epub 2017 Dec 14.
3
Downregulation of can enhance the radiosensitivity of osteosarcoma and .下调 可以增强骨肉瘤 和 的放射敏感性。
Epigenomics. 2019 Aug;11(11):1283-1305. doi: 10.2217/epi-2019-0125. Epub 2019 Jul 29.
4
Knockdown of telomeric repeat binding factor 2 enhances tumor radiosensitivity regardless of telomerase status.端粒重复序列结合因子2的敲低可增强肿瘤放射敏感性，而与端粒酶状态无关。
J Cancer Res Clin Oncol. 2015 Sep;141(9):1545-52. doi: 10.1007/s00432-015-1911-8. Epub 2015 Jan 22.
5
The downregulation of miR-144 is associated with the growth and invasion of osteosarcoma cells through the regulation of TAGLN expression.miR-144的下调通过调控TAGLN的表达与骨肉瘤细胞的生长和侵袭相关。
Int J Mol Med. 2014 Dec;34(6):1565-72. doi: 10.3892/ijmm.2014.1963. Epub 2014 Oct 10.
6
miR-223-3p Inhibits Human Osteosarcoma Metastasis and Progression by Directly Targeting CDH6.miR-223-3p 通过直接靶向 CDH6 抑制人骨肉瘤转移和进展。
Mol Ther. 2018 May 2;26(5):1299-1312. doi: 10.1016/j.ymthe.2018.03.009. Epub 2018 Mar 14.
7
Effect of miRNA-200a on radiosensitivity of osteosarcoma cells by targeting Bone morphogenetic protein receptor 2.miRNA-200a 通过靶向骨形态发生蛋白受体 2 对骨肉瘤细胞放射敏感性的影响。
Bioengineered. 2021 Dec;12(2):12625-12635. doi: 10.1080/21655979.2021.2011015.
8
The miR-15b-5p/PDK4 axis regulates osteosarcoma proliferation through modulation of the Warburg effect.miR-15b-5p/PDK4 轴通过调节瓦博格效应调控骨肉瘤增殖。
Biochem Biophys Res Commun. 2018 Sep 18;503(4):2749-2757. doi: 10.1016/j.bbrc.2018.08.035. Epub 2018 Aug 6.
9
MiR-26a inhibits stem cell-like phenotype and tumor growth of osteosarcoma by targeting Jagged1.miR-26a 通过靶向 Jagged1 抑制骨肉瘤干细胞样表型和肿瘤生长。
Oncogene. 2017 Jan 12;36(2):231-241. doi: 10.1038/onc.2016.194. Epub 2016 Jun 6.
10
MicroRNA-187 Inhibits Growth and Metastasis of Osteosarcoma by Downregulating S100A4.微小RNA-187通过下调S100A4抑制骨肉瘤的生长和转移。
Cancer Invest. 2018 Jan 2;36(1):1-9. doi: 10.1080/07357907.2017.1415348. Epub 2018 Jan 5.

引用本文的文献

1
The Landscape of microRNAs in Bone Tumor: A Comprehensive Review in Recent Studies.骨肿瘤 microRNAs 全景：近期研究全面综述。
Microrna. 2024;13(3):175-201. doi: 10.2174/0122115366298799240625115843.
2
Radiotherapy in bone sarcoma: the quest for better treatment option.骨肉瘤的放射治疗：寻求更好的治疗选择。
BMC Cancer. 2023 Aug 11;23(1):742. doi: 10.1186/s12885-023-11232-3.
3
Non-coding RNAs in drug and radiation resistance of bone and soft-tissue sarcoma: a systematic review.非编码 RNA 在骨与软组织肉瘤的药物和放射抵抗中的作用：一项系统综述。

本文引用的文献

1
Loss of MicroRNA-489-3p promotes osteosarcoma metastasis by activating PAX3-MET pathway.MicroRNA-489-3p缺失通过激活PAX3-MET通路促进骨肉瘤转移。
Mol Carcinog. 2017 Apr;56(4):1312-1321. doi: 10.1002/mc.22593. Epub 2016 Nov 29.
2
miR-424 acts as a tumor radiosensitizer by targeting aprataxin in cervical cancer.在宫颈癌中，miR-424通过靶向 aprataxin发挥肿瘤放射增敏剂的作用。
Oncotarget. 2016 Nov 22;7(47):77508-77515. doi: 10.18632/oncotarget.12716.
3
[miR-124 regulates radiosensitivity of colorectal cancer cells by targeting PRRX1].
Elife. 2022 Nov 3;11:e79655. doi: 10.7554/eLife.79655.
4
MicroRNA miR-331-3p suppresses osteosarcoma progression via the Bcl-2/Bax and Wnt/β-Catenin signaling pathways and the epithelial-mesenchymal transition by targeting N-acetylglucosaminyltransferase I (MGAT1).微小 RNA miR-331-3p 通过靶向 N-乙酰氨基葡萄糖转移酶 I（MGAT1）抑制骨肉瘤进展，其作用机制与 Bcl-2/Bax 和 Wnt/β-连环蛋白信号通路以及上皮-间充质转化有关。
Bioengineered. 2022 Jun;13(6):14159-14174. doi: 10.1080/21655979.2022.2083855.
5
c-Myb-mediated inhibition of miR-601 in facilitating malignance of osteosarcoma via augmentation of PKMYT1.c-Myb 介导的 miR-601 抑制通过增强 PKMYT1 促进骨肉瘤的恶性转化。
Sci Rep. 2022 Apr 23;12(1):6692. doi: 10.1038/s41598-022-10684-0.
6
Hsa-miR-557 Inhibits Osteosarcoma Growth Through Targeting KRAS.人源微小RNA-557通过靶向KRAS抑制骨肉瘤生长。
Front Genet. 2022 Jan 11;12:789823. doi: 10.3389/fgene.2021.789823. eCollection 2021.
7
miR-328-3p promotes migration and invasion by targeting H2AFX in head and neck squamous cell carcinoma.miR-328-3p通过靶向H2AFX促进头颈部鳞状细胞癌的迁移和侵袭。
J Cancer. 2021 Sep 9;12(21):6519-6530. doi: 10.7150/jca.60743. eCollection 2021.
8
LncRNA SNHG17 promotes tumor progression and predicts poor survival in human renal cell carcinoma via sponging miR-328-3p.长链非编码 RNA SNHG17 通过海绵吸附 miR-328-3p 促进人肾细胞癌的肿瘤进展并预测不良预后。
Aging (Albany NY). 2021 Sep 8;13(17):21232-21250. doi: 10.18632/aging.203440.
9
Myocardial infarction cardiomyocytes-derived exosomal miR-328-3p promote apoptosis via Caspase signaling.心肌梗死来源的心肌细胞外泌体miR-328-3p通过半胱天冬酶信号通路促进细胞凋亡。
Am J Transl Res. 2021 Apr 15;13(4):2365-2378. eCollection 2021.
10
MiR-183-5p Promotes Tumor Progression of Osteosarcoma and Predicts Poor Prognosis in Patients.微小RNA-183-5p促进骨肉瘤肿瘤进展并预示患者预后不良。
Cancer Manag Res. 2021 Jan 27;13:805-814. doi: 10.2147/CMAR.S285909. eCollection 2021.
[微小RNA-124通过靶向脯氨酸丰富的同源框1调节结肠癌细胞的放射敏感性]
Nan Fang Yi Ke Da Xue Xue Bao. 2016 Aug 20;36(8):1110-6.
4
HLF/miR-132/TTK axis regulates cell proliferation, metastasis and radiosensitivity of glioma cells.HLF/miR-132/TTK 轴调控胶质瘤细胞的增殖、转移和放射敏感性。
Biomed Pharmacother. 2016 Oct;83:898-904. doi: 10.1016/j.biopha.2016.08.004. Epub 2016 Aug 10.
5
MiR-328 targeting PIM-1 inhibits proliferation and migration of pulmonary arterial smooth muscle cells in PDGFBB signaling pathway.靶向PIM-1的MiR-328抑制血小板衍生生长因子BB信号通路中肺动脉平滑肌细胞的增殖和迁移。
Oncotarget. 2016 Aug 23;7(34):54998-55011. doi: 10.18632/oncotarget.10714.
6
Upregulation of microRNA-98 increases radiosensitivity in esophageal squamous cell carcinoma.微小RNA-98的上调增加食管鳞状细胞癌的放射敏感性。
J Radiat Res. 2016 Sep;57(5):468-476. doi: 10.1093/jrr/rrw068. Epub 2016 Jul 15.
7
miR-494-3p Induces Cellular Senescence and Enhances Radiosensitivity in Human Oral Squamous Carcinoma Cells.miR-494-3p诱导人口腔鳞状癌细胞发生细胞衰老并增强其放射敏感性。
Int J Mol Sci. 2016 Jul 8;17(7):1092. doi: 10.3390/ijms17071092.
8
MicroRNA-187 inhibits tumor growth and invasion by directly targeting CD276 in colorectal cancer.微小RNA-187通过直接靶向结直肠癌中的CD276抑制肿瘤生长和侵袭。
Oncotarget. 2016 Jul 12;7(28):44266-44276. doi: 10.18632/oncotarget.10023.
9
miR-124 radiosensitizes human esophageal cancer cell TE-1 by targeting CDK4.微小RNA-124通过靶向细胞周期蛋白依赖性激酶4使人类食管癌细胞TE-1对放疗敏感。
Genet Mol Res. 2016 Jun 3;15(2):gmr7893. doi: 10.4238/gmr.15027893.
10
MicroRNA-449a enhances radiosensitivity by downregulation of c-Myc in prostate cancer cells.微小RNA-449a通过下调前列腺癌细胞中的c-Myc增强放射敏感性。
Sci Rep. 2016 Jun 2;6:27346. doi: 10.1038/srep27346.