Department of Energy, Environmental and Chemical Engineering, Washington University in St. Louis, Saint Louis, MO 63130, USA.
Department of Energy, Environmental and Chemical Engineering, Washington University in St. Louis, Saint Louis, MO 63130, USA; Division of Biological & Biomedical Sciences, Washington University in St. Louis, Saint Louis, MO 63130, USA; Institute of Materials Science & Engineering, Washington University in St. Louis, Saint Louis, MO 63130, USA.
Curr Opin Biotechnol. 2018 Oct;53:26-32. doi: 10.1016/j.copbio.2017.10.010. Epub 2017 Dec 5.
The past decade has witnessed rapid advance in microbial production of 'drop-in' biofuels from renewable resources. Various biosynthetic pathways have been constructed to produce biofuels with diverse structures, and multiple metabolic engineering strategies have been developed to increase biofuel titers, yields, productivities and system robustness. In this review, we intend to give a brief but comprehensive overview of the most recent progresses on four essential pathways leading to 'drop-in' biofuel production, with an emphasis on the metabolic pathway efficiencies and biofuel structures. Furthermore, we also provide an insightful discussion on optimization strategies to improve the robustness of the microbial platforms for biofuel production.
过去十年见证了微生物利用可再生资源生产“可替代”生物燃料的快速发展。已经构建了各种生物合成途径来生产具有不同结构的生物燃料，并且已经开发了多种代谢工程策略来提高生物燃料的产率、产量、生产力和系统稳定性。在这篇综述中，我们旨在简要但全面地概述通向“可替代”生物燃料生产的四条主要途径的最新进展，重点是代谢途径效率和生物燃料结构。此外，我们还就提高生物燃料生产微生物平台的稳定性的优化策略进行了深入讨论。
