Zargar Amin, Bailey Constance B, Haushalter Robert W, Eiben Christopher B, Katz Leonard, Keasling Jay D
Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Emeryville, CA 94608, United States; QB3 Institute, University of California-Berkeley, 5885 Hollis Street, 4th Floor, Emeryville, CA 94608, United States.
Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Emeryville, CA 94608, United States; Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, United States.
Curr Opin Biotechnol. 2017 Jun;45:156-163. doi: 10.1016/j.copbio.2017.03.004. Epub 2017 Apr 17.
Advances in retooling microorganisms have enabled bioproduction of 'drop-in' biofuels, fuels that are compatible with existing spark-ignition, compression-ignition, and gas-turbine engines. As the majority of petroleum consumption in the United States consists of gasoline (47%), diesel fuel and heating oil (21%), and jet fuel (8%), 'drop-in' biofuels that replace these petrochemical sources are particularly attractive. In this review, we discuss the application of aldehyde decarbonylases to produce gasoline substitutes from fatty acid products, a recently crystallized reductase that could hydrogenate jet fuel precursors from terpene synthases, and the exquisite control of polyketide synthases to produce biofuels with desired physical properties (e.g., lower freezing points). With our increased understanding of biosynthetic logic of metabolic pathways, we discuss the unique advantages of fatty acid, terpene, and polyketide synthases for the production of bio-based gasoline, diesel and jet fuel.
对微生物进行改造的进展使得“直接可用”生物燃料的生物生产成为可能，这类燃料可与现有的火花点火式、压缩点火式和燃气轮机发动机兼容。由于美国大部分石油消费包括汽油（47%）、柴油和取暖油（21%）以及喷气燃料（8%），因此替代这些石化来源的“直接可用”生物燃料格外具有吸引力。在本综述中，我们讨论了醛脱羰酶在从脂肪酸产物生产汽油替代品方面的应用、一种最近结晶的还原酶，该酶可将萜烯合酶产生的喷气燃料前体氢化，以及聚酮合酶的精确控制以生产具有所需物理性质（如较低冰点）的生物燃料。随着我们对代谢途径生物合成逻辑的深入理解，我们讨论了脂肪酸、萜烯和聚酮合酶在生产生物基汽油、柴油和喷气燃料方面的独特优势。
