Xu Xiao-Wu, Zheng Bo-An, Hu Zhi-Ming, Qian Zhen-Yuan, Huang Chao-Jie, Liu Xi-Qiang, Wu Wei-Ding
Department of Gastrointestinal and Pancreatic Surgery, Zhejiang Provincial People's Hospital, Key Laboratory of Gastroenterology of Zhejiang Provincial People's Hospital of Hangzhou Medical College, Hangzhou, 310014, Zhejiang, China.
Department of Colorectal Surgery, Zhejiang Provincial People's Hospital, Hangzhou, 310014, Zhejiang, China.
Oncotarget. 2017 Oct 10;8(53):91674-91683. doi: 10.18632/oncotarget.21748. eCollection 2017 Oct 31.
Circular RNAs (circRNAs) as a novel type of noncoding RNAs (ncRNAs) are widely studied in the development of human various diseases, including cancer. Here, we found circular RNA hsa_circ_000984 encoded by the gene was remarkably upregulated in the tissues of colorectal cancer (CRC) patients and in the CRC cell lines. Moreover, high expression level of hsa_circ_000984 was significantly associated with advanced colorectal cancer. Further analysis revealed that hsa_circ_000984 knockdown could inhibit cell proliferation, migration, invasion and tumor formation in CRC cell lines. Mechanically, we found that hsa_circ_000984 may act as a competing endogenous RNA (ceRNA) by competitively binding miR-106b and effectively upregulate the expression of , thereby inducing a series of malignant phenotypes of tumor cells. Taken together, these observations suggest that the hsa_circ_000984 could mediate the expression of gene by acting as a ceRNA, which may contribute to a better understanding of between the regulatory miRNA network and CRC pathogenesis.
环状RNA（circRNAs）作为一种新型的非编码RNA（ncRNAs），在包括癌症在内的人类多种疾病的发展过程中受到广泛研究。在此，我们发现由该基因编码的环状RNA hsa_circ_000984在结直肠癌（CRC）患者的组织以及CRC细胞系中显著上调。此外，hsa_circ_000984的高表达水平与晚期结直肠癌显著相关。进一步分析表明，敲低hsa_circ_000984可抑制CRC细胞系中的细胞增殖、迁移、侵袭及肿瘤形成。从机制上讲，我们发现hsa_circ_000984可能通过竞争性结合miR-106b作为一种竞争性内源性RNA（ceRNA），并有效上调 的表达，从而诱导肿瘤细胞的一系列恶性表型。综上所述，这些观察结果表明hsa_circ_000984可作为ceRNA介导基因的表达，这可能有助于更好地理解调控性miRNA网络与CRC发病机制之间的关系。
