Zhu Chang-Li, Sha Xiaofeng, Wang Yuan, Li Jin, Zhang Men-Yan, Guo Zhong-Ying, Sun Su-An, He Jing-Dong
Department of Medical Oncology, Huai'an First People's Hospital, Nanjing Medical University, Huai'an City, Jiangsu Province 223300, China.
Department of Oncology, People's Hospital of Hongze District, Huai'an City, Jiangsu Province 223300, China.
J Oncol. 2019 Jun 26;2019:9836819. doi: 10.1155/2019/9836819. eCollection 2019.
Circular RNAs (circRNAs) are a large class of endogenous noncoding RNAs that regulate gene expression and mainly function as microRNA sponges. This study aimed to explore the aberrant expression of circRNAs in colorectal cancer (CRC). Using a circRNA microarray, we identified 892 differentially expressed circRNAs between six pairs of CRC and adjacent paracancerous tissues. Among them, hsa_circ_0007142 was significantly upregulated. Further analysis in 50 CRC clinical samples revealed that hsa_circ_0007142 upregulation was associated with poor differentiation and lymphatic metastasis of CRC. Bioinformatic analysis and luciferase reporter assay showed that hsa_circ_0007142 targeted miR-103a-2-5p in CRC cells. Moreover, the silencing of hsa_circ_0007142 by siRNAs decreased the proliferation, migration, and invasion of HT-29 and HCT-116 cells. Taken together, these findings suggest that hsa_circ_0007142 is upregulated in CRC and targets miR-103a-2-5p to promote CRC.
环状RNA（circRNAs）是一大类内源性非编码RNA，可调节基因表达，主要作为微小RNA海绵发挥作用。本研究旨在探讨环状RNA在结直肠癌（CRC）中的异常表达。通过环状RNA微阵列，我们在六对结直肠癌组织和相邻癌旁组织中鉴定出892个差异表达的环状RNA。其中，hsa_circ_0007142显著上调。对50例结直肠癌临床样本的进一步分析表明，hsa_circ_0007142的上调与结直肠癌的低分化和淋巴转移相关。生物信息学分析和荧光素酶报告基因检测表明，hsa_circ_0007142在结直肠癌细胞中靶向miR-103a-2-5p。此外，通过小干扰RNA沉默hsa_circ_0007142可降低HT-29和HCT-116细胞的增殖、迁移和侵袭。综上所述，这些发现表明hsa_circ_0007142在结直肠癌中上调，并靶向miR-103a-2-5p以促进结直肠癌的发生发展。
