Cim Numan, Tolunay Harun Egemen, Karaman Erbil, Boza Barıs, Bilici Mustafa, Çetin Orkun, Yıldızhan Recep, Sahin Hanım Guler
1 Yuzuncu Yil University Medical Faculty, Department of Obstetrics and Gynecology, Van, Turkey.
2 Yuzuncu Yil University, Van Security Collage, Department of Biochemistry, Van, Turkey.
J Int Med Res. 2018 Mar;46(3):1146-1152. doi: 10.1177/0300060517734443. Epub 2017 Dec 6.
Objective This study aimed to evaluate the oxidant-antioxidant status of amniotic fluid in pregnant women with foetal congenital malformations of the central nervous system. Methods We studied pregnant women with foetal congenital nervous system anomalies at 16-22 weeks' gestation (n = 36). The control group (n = 30) consisted of pregnant women at the same gestational age who underwent amniocentesis, resulting in a normal karyotype. We analysed glutathione, catalase, and malondialdehyde levels in amniotic fluid. Enzyme activation was measured by spectrophotometry. Results The demographic features of the groups were similar in terms of age, parity, body mass index, and gestational weeks. We detected lower glutathione and catalase levels in the foetal congenital anomaly group than in the control group. We detected higher malondialdehyde levels in the foetal congenital anomaly group than in the control group. Conclusion In the organism, the rate of formation of free radicals and their rate of removal are balanced, and this is called oxidative balance. As long as oxidative stability is achieved, the organism is not affected by free radicals. This fact should be kept in mind to avoid any type of teratogenic agent that could lead to congenital disorders.
目的 本研究旨在评估胎儿中枢神经系统先天性畸形孕妇羊水的氧化-抗氧化状态。方法 我们研究了妊娠16 - 22周的胎儿先天性神经系统异常孕妇（n = 36）。对照组（n = 30）由相同孕周行羊膜腔穿刺且核型正常的孕妇组成。我们分析了羊水中谷胱甘肽、过氧化氢酶和丙二醛水平。酶活性通过分光光度法测定。结果 两组在年龄、产次、体重指数和孕周方面的人口统计学特征相似。我们检测到胎儿先天性异常组的谷胱甘肽和过氧化氢酶水平低于对照组。我们检测到胎儿先天性异常组的丙二醛水平高于对照组。结论 在生物体中，自由基的形成速率与其清除速率是平衡的，这被称为氧化平衡。只要实现氧化稳定性，生物体就不会受到自由基的影响。应牢记这一事实，以避免任何可能导致先天性疾病的致畸剂。
