Department of Animal Production, Henan University of Science and Technology, Luoyang 471003, China.
Department of Poultry Science, University of Georgia, Athens 30602, GA, USA.
Poult Sci. 2018 Feb 1;97(2):477-484. doi: 10.3382/ps/pex342.
This study aimed to investigate the effect of yeast cell walls (YCW) on the growth performance, visceral lesions, intestinal integrity, enterotoxicity, and bacteria of broilers challenged with aflatoxin B1 (AF) and necrotic enteritis (NE) from 1 to 21 d of age. A total of 576 one-day-old broilers were assigned to a 2 × 2 × 2 design for diets containing AFB1 (0 or 40 μg/kg), NE (challenged or unchallenged), or YCW (0 or 500 mg/kg). The main effect analysis showed that AF depressed (P < 0.01) average daily feed intake (ADFI), average daily body weight gain (ADG), the mRNA profiles of polymeric Ig receptor (pIgR), claudin-1, and occludin, but increased (P < 0.001) liver lesion scores, serum endotoxin, and diamine oxidase (DAO). The NE challenge depressed (P < 0.01) ADFI, ADG, secretory IgA (sIgA), pIgR, claudin-1, occludin, and the populations of Lactobacilli and Bifidobacteria, but increased (P < 0.001) visceral lesions, endotoxins, and DAO. The main effect of YCW on growth performance, visceral lesions, and intestinal integrity was not significant, but decreased (P < 0.01) mortality, endotoxin, DAO, and C. perfringens, and increased (P < 0.05) the populations of Lactobacilli and Bifidobacteria. There were 3-way interactions (P < 0.05) on growth performance, intestinal lesions, integrity, and gut bacteria. Compared with the treatment with the dual challenges, there were pronounced effects (P < 0.05) of YCW on ADFI, ADG, lesions, DAO, pIgR, and Bifidobacteria. The results suggest that with the concurrent challenges of AF and NE, the YCW can partially protect the growth performance and intestinal health of broilers.
本研究旨在探讨酵母细胞壁（YCW）对 1 至 21 日龄受黄曲霉毒素 B1（AF）和坏死性肠炎（NE）挑战的肉鸡生长性能、内脏损伤、肠道完整性、肠毒性和细菌的影响。总共 576 只 1 日龄的肉鸡被分配到 2×2×2 的设计中，饲料中含有 AFB1（0 或 40μg/kg）、NE（挑战或不挑战）或 YCW（0 或 500mg/kg）。主效应分析表明，AF 降低（P<0.01）平均日采食量（ADFI）、平均日增重（ADG）、多聚免疫球蛋白受体（pIgR）、闭合蛋白-1 和闭合蛋白-4 的 mRNA 谱，但增加（P<0.001）肝损伤评分、血清内毒素和二胺氧化酶（DAO）。NE 挑战降低（P<0.01）ADFI、ADG、分泌型 IgA（sIgA）、pIgR、闭合蛋白-1、闭合蛋白-4 和乳杆菌和双歧杆菌的数量，但增加（P<0.001）内脏损伤、内毒素和 DAO。YCW 对生长性能、内脏损伤和肠道完整性的主要影响不显著，但降低（P<0.01）死亡率、内毒素、DAO 和产气荚膜梭菌，并增加（P<0.05）乳杆菌和双歧杆菌的数量。生长性能、肠道损伤、完整性和肠道细菌存在 3 种方式的相互作用（P<0.05）。与双重挑战的处理相比，YCW 对 ADFI、ADG、病变、DAO、pIgR 和双歧杆菌有明显的影响（P<0.05）。结果表明，在同时受到 AF 和 NE 挑战的情况下，YCW 可以部分保护肉鸡的生长性能和肠道健康。
