Effects of graded yeast cell wall supplementation on growth performance, immunity and intestinal development of broiler chickens raised in floor pens for 42 days.

This study was conducted to evaluate the effects of dietary supplementation of a novel soluble yeast cell wall (YCW) on growth performance, gut health, intestinal morphology, and immune response in broiler chickens for 42 days. A total of 480 one-day-old Cobb 500 male broilers were randomly assigned to four treatments with six replicates and each replicate of twenty broiler chickens: a control group (CON) without feed additive supplementation, and three groups supplemented with YCW at 0.025 % (YCW1), 0.050 % (YCW2), and 0.100 % (YCW3). Results showed that 0.025 % and 0.100 % YCW supplementation significantly increased (P < 0.05) final body weight (BW) and overall body weight gain (BWG) while reducing overall feed conversion ratio (FCR) compared to the CON group. The YCW supplementation also improved (P < 0.05) the balance of gut microbiota by increasing beneficial bacteria (Lactobacillus) and decreasing Salmonella, a potential foodborne pathogen in humans in the ceca. Although intestinal morphology was not significantly affected, YCW supplementation numerically increased the villus height: crypt depth ratio (VH:CD) compared to the CON group. Furthermore, YCW reduced the mRNA expression of pro-inflammatory cytokines (IL-1β and INF-γ) and tight junction protein claudin-1 (CLDN-1) (P < 0.05), suggesting balanced immune response and improved intestinal barrier function. In conclusion, the supplementation of soluble YCW in broiler diets positively influenced growth performance, gut microbiota composition, and immune response, demonstrating its potential as a viable alternative to antibiotics for improving broilers' health.