生物素标记抗原筛选法检测弓形虫IgM抗体
Biotin-labelled antigen screening test for toxoplasma IgM antibody.
作者信息
Joss A W, Skinner L J, Moir I L, Chatterton J M, Williams H, Ho-Yen D O
机构信息
Scottish Toxoplasma Reference Laboratory, Raigmore Hospital, Inverness, Scotland.
出版信息
J Clin Pathol. 1989 Feb;42(2):206-9. doi: 10.1136/jcp.42.2.206.
Abstract
A method for the simple preparation of biotin-labelled toxoplasma antigen was used with avidin peroxidase in an IgM-capture enzyme linked immunosorbent assay (BAM-ELISA). Although the overall predictive value of a positive result was only 38%, its low cost and 100% sensitivity makes it a very suitable screening test. Positive results can be confirmed by an alternative assay, thus providing a more economical and effective diagnostic service than either screening all sera by a commercial test or selecting sera for IgM testing.
摘要
一种用于简单制备生物素标记的弓形虫抗原的方法与抗生物素蛋白过氧化物酶一起用于IgM捕获酶联免疫吸附测定（BAM-ELISA）。尽管阳性结果的总体预测价值仅为38%，但其低成本和100%的灵敏度使其成为一种非常合适的筛查试验。阳性结果可以通过另一种检测方法进行确认，因此比通过商业检测筛查所有血清或选择血清进行IgM检测提供了更经济有效的诊断服务。
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