Simonsson T
Department of Oral Microbiology, Faculty of Odontology, University of Lund, Malmö, Sweden.
Swed Dent J Suppl. 1989;58:1-67.
In order to investigate factors of importance for plaque formation and the rate of plaque formation 133 randomly selected individuals went through a 3-day period of plaque accumulation. From these individuals one group of "heavy" and one group of "light" plaque formers were selected for further studies. These subjects were investigated with reference to clinical, biochemical, biophysical and microbiological variables, that in the literature have been suggested to influence plaque formation. The collected data were analysed statistically both by comparisons between the two groups and by multiple regression. In the comparative analyses there were only minor differences between the groups, and no single studied variable was considered as the only explanation to the great difference in the amount of plaque formed after 3 days between "heavy" and "light" plaque formers. The multiple regression showed that the initial bacterial colonization of tooth surfaces was dependent on the clinical wettability of tooth surfaces, the saliva-induced aggregation of oral bacteria and finally the relative salivary flow conditions around the tooth surfaces where the bacterial samples were collected. On basis of these results, it was suggested that saliva and oral bacteria could at least partly be looked upon as being a biological colloid system system. Further studies were designed to investigate whether oral bacteria could be regarded as colloid particles or not, and to study the properties of saliva as a colloid suspending medium. These studies showed that bacteria suspended in different salt solutions were dependent on pH, ionic concentration and the valency of the cations in the solutions to be able to aggregate. The aggregating capacity further seemed to be specific for different bacterial species and strains. Addition of saliva from "heavy" and "light" plaque formers influenced the colloid stability of bacteria suspended in water in different ways. Saliva from "light" plaque formers gave a lower colloid stability in suspensions of a plaque-forming bacterium (Streptococcus sanguis) as compared to the addition of saliva from "heavy" plaque formers. No such differences were observed in suspensions of S. salivarius, which is normally not a plaque-forming bacterium. The results indicate that colloid-chemical processes might be involved in bacterial attachment to tooth surfaces, and that initial bacterial colonization of tooth surfaces at least partly follow general biophysical laws.
为了研究对牙菌斑形成及牙菌斑形成速率具有重要意义的因素，133名随机选取的个体经历了为期3天的牙菌斑积累期。从这些个体中，挑选出一组“重度”牙菌斑形成者和一组“轻度”牙菌斑形成者进行进一步研究。针对临床、生化、生物物理和微生物学变量对这些受试者进行了调查，这些变量在文献中被认为会影响牙菌斑形成。收集到的数据通过两组之间的比较以及多元回归进行了统计分析。在比较分析中，两组之间只有细微差异，而且没有任何一个研究变量被视为对“重度”和“轻度”牙菌斑形成者3天后牙菌斑形成量巨大差异的唯一解释。多元回归表明，牙面的初始细菌定植取决于牙面的临床润湿性、唾液诱导的口腔细菌聚集，以及最终收集细菌样本的牙面周围的相对唾液流动状况。基于这些结果，有人提出唾液和口腔细菌至少在一定程度上可被视为一个生物胶体系统。进一步的研究旨在调查口腔细菌是否可被视为胶体颗粒，并研究唾液作为胶体悬浮介质的特性。这些研究表明，悬浮在不同盐溶液中的细菌能否聚集取决于溶液的pH值、离子浓度和阳离子的价态。聚集能力似乎还因不同的细菌种类和菌株而异。添加“重度”和“轻度”牙菌斑形成者的唾液对悬浮在水中的细菌的胶体稳定性有不同影响。与添加“重度”牙菌斑形成者的唾液相比，添加“轻度”牙菌斑形成者的唾液会使一种致龋细菌（血链球菌）悬浮液中的胶体稳定性降低。在唾液链球菌悬浮液中未观察到此类差异，唾液链球菌通常不是致龋细菌。结果表明，胶体化学过程可能参与了细菌在牙面的附着，并且牙面的初始细菌定植至少部分遵循一般生物物理规律。
