对 Saprochaete capitata 的热点 1 进行测序以寻找与降低棘白菌素敏感性的关系。
Sequencing of Hot Spot 1 from Saprochaete capitata To Search for a Relationship to Reduced Echinocandin Susceptibility.
机构信息
Department of Nursing, Faculty of Medicine and Nursing, University of the Basque Country UPV/EHU, Leioa, Bizkaia, Spain.
Department of Immunology, Microbiology and Parasitology, Faculty of Medicine and Nursing, University of the Basque Country UPV/EHU, Leioa, Bizkaia, Spain.
出版信息
Antimicrob Agents Chemother. 2018 Jan 25;62(2). doi: 10.1128/AAC.01246-17. Print 2018 Feb.
Abstract
, formerly known as , is an emerging fungal pathogen with low susceptibility to echinocandins. Here, we report the nucleotide sequence of the hot spot 1 region of the gene ( HS1), which codifies for the catalytic subunit of β-1,3-d-glucan synthase, the target of echinocandins. For that purpose, we first designed degenerated oligonucleotide primers derived from conserved flanking regions of the HS1 segment of 12 different fungal species. Interestingly, analysis of the translated HS1 sequences of 12 isolates of revealed that all of them exhibited the same F-to-L substitution in a position that is highly related to reduced echinocandin susceptibility.
摘要
棘白菌素类药物低敏感性的新兴真菌病原体。在这里，我们报道了编码棘白菌素类药物靶位β-1,3-d-葡聚糖合酶催化亚基的 基因（ HS1）热点 1 区的核苷酸序列。为此，我们首先设计了源自 12 种不同真菌物种的 HS1 片段侧翼保守区的简并寡核苷酸引物。有趣的是，对 12 株 分离株的 HS1 序列分析表明，它们在一个与棘白菌素类药物敏感性降低高度相关的位置都表现出相同的 F 到 L 取代。
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2
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Curr Opin Infect Dis. 2014 Dec;27(6):484-92. doi: 10.1097/QCO.0000000000000111.
3
Name changes in medically important fungi and their implications for clinical practice.
J Clin Microbiol. 2015 Apr;53(4):1056-62. doi: 10.1128/JCM.02016-14. Epub 2014 Oct 8.
4
Invasive infections caused by Saprochaete capitata in patients with haematological malignancies: report of five cases and review of the antifungal therapy.
Rev Iberoam Micol. 2013 Oct-Dec;30(4):248-55. doi: 10.1016/j.riam.2013.02.004. Epub 2013 Apr 11.
5
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J Antimicrob Chemother. 2012 Jul;67(7):1666-76. doi: 10.1093/jac/dks112. Epub 2012 Apr 18.
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Assessment of two new molecular methods for identification of Candida parapsilosis sensu lato species.
J Clin Microbiol. 2011 Sep;49(9):3257-61. doi: 10.1128/JCM.00508-11. Epub 2011 Jul 27.
7
Evaluation of caspofungin or micafungin as empiric antifungal therapy in adult patients with persistent febrile neutropenia: a retrospective, observational, sequential cohort analysis.
Clin Ther. 2010 Apr;32(4):637-48. doi: 10.1016/j.clinthera.2010.04.005.
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