Department of Orthopaedic Surgery, Sir Run Run Shaw Hospital, Medical College of Zhejiang University, Hangzhou, China.
Department of Pathophysiology, Wenzhou Medical University, Wenzhou, China.
Osteoarthritis Cartilage. 2018 Mar;26(3):433-444. doi: 10.1016/j.joca.2017.12.001. Epub 2017 Dec 9.
To investigate the effect of decellularized osteochondral extracellular matrix (ECM) scaffold for osteochondral defect regeneration.
We compared the histological features and microstructure of degenerated cartilage to normal articular cartilage. We also generated and evaluated osteochondral ECM scaffolds through decellularization technology. Then scaffolds were implanted to osteochondral defect in rabbit model. After 12 weeks surgery, regeneration tissues were analyzed by histology, immunohistochemistry evaluation. And possible mechanisms of angiogenesis and cell migration were explored.
We demonstrated decreased cell numbers, formation of fibrous cartilage, lost microstructure and worse permeability in degenerated cartilage compared to normal cartilage. We also generated an osteochondral ECM scaffold with a hierarchical structure that exhibited low immunogenicity, high bioactivity, and well biocompatibility. We found that the ECM scaffold promoted tissue regeneration in osteochondral defects, which was dependent on the scaffold constituents and stratified three-dimensional microstructure as well as on its ability to inhibit angiogenesis and stimulate cell migration.
Our findings demonstrated that the biphasic hierarchical ECM scaffold represents a novel and effective biomaterial that can be used in the treatment of osteochondral defect.
研究去细胞化的骨软骨细胞外基质（ECM）支架在骨软骨缺损再生中的作用。
我们比较了退变软骨和正常关节软骨的组织学特征和微观结构。我们还通过去细胞化技术生成和评估了骨软骨 ECM 支架。然后将支架植入兔骨软骨缺损模型中。术后 12 周，通过组织学、免疫组织化学评估分析再生组织。并探讨了血管生成和细胞迁移的可能机制。
与正常软骨相比，退变软骨中细胞数量减少，形成纤维软骨，微观结构丢失，通透性变差。我们还生成了一种具有分层结构的骨软骨 ECM 支架，表现出低免疫原性、高生物活性和良好的生物相容性。我们发现 ECM 支架促进了骨软骨缺损中的组织再生，这依赖于支架成分和分层的三维微观结构，以及其抑制血管生成和刺激细胞迁移的能力。
我们的研究结果表明，双相分层 ECM 支架是一种新型有效的生物材料，可用于治疗骨软骨缺损。
