Guo Di, Song Xiaoming, Yuan Min, Wang Zhenyi, Ge Weina, Wang Li, Wang Jinpeng, Wang Xiyin
School of Life Sciences, North China University of Science and Technology, Tangshan, China.
Center for Genomics and Computational Biology, North China University of Science and Technology, Tangshan, China.
Front Plant Sci. 2017 Nov 28;8:2001. doi: 10.3389/fpls.2017.02001. eCollection 2017.
Plants growing under high-density (HD) conditions experience increased competition for water, nutrients, and light, possibly leading to changes in size, biomass, morphology, and productivity. However, no research has focused on the relationship between whole-genome expression patterns and growth density. Here, we performed whole-genome RNA sequencing to examine the gene expression patterns in grown under low and high densities. Of the 20,660 detected genes, the expression levels of 98 were enhanced and 107 were repressed under HD growth. Further analysis revealed that changes in density influenced metabolism- and stimulus-related genes the most. Furthermore, HD growth led to a shade avoidance phenotype, represented by upward growth and a reduction in rosette leaves. Moreover, a cluster of glutaredoxin genes, , and , were significantly down-regulated under high density, suggesting that high density affects plant growth mainly by nitrate limitation.
在高密度（HD）条件下生长的植物，对水、养分和光照的竞争加剧，这可能导致植物在大小、生物量、形态和生产力方面发生变化。然而，尚未有研究聚焦于全基因组表达模式与生长密度之间的关系。在此，我们进行了全基因组RNA测序，以检测在低密度和高密度条件下生长的植物的基因表达模式。在检测到的20660个基因中，有98个基因的表达水平在高密度生长条件下增强，107个基因的表达水平受到抑制。进一步分析表明，密度变化对与代谢和刺激相关的基因影响最大。此外，高密度生长导致了避荫表型，表现为向上生长和莲座叶减少。此外，一组谷氧还蛋白基因，即、和，在高密度条件下显著下调，这表明高密度主要通过硝酸盐限制影响植物生长。
