Prabhakaran Aneesh, Hamid Ahmed M, Garimella Sandilya V B, Valenzuela Blandina R, Ewing Robert G, Ibrahim Yehia M, Smith Richard D
Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, 99352, USA.
J Am Soc Mass Spectrom. 2018 Feb;29(2):342-351. doi: 10.1007/s13361-017-1841-6. Epub 2017 Dec 12.
Here we explore the combination of constant and oscillatory fields applied in a single device to affect the continuous separation and filtering of ions based on their mobilities. The device explored allows confining and manipulating ions utilizing a combination of radio frequency (rf), direct current (DC) fields, and traveling waves (TW) in a structures for lossless ion manipulations (SLIM) module. We have investigated theoretically and experimentally a concept for continuous filtering of ions based on their mobilities where ions are mobility separated and selected by passage through two regions, both of which incorporated combined TW and constant fields providing opposing forces on the ions. The SLIM module was composed of two surfaces with mirror-image arrays of electrodes and had two regions where the different TW and opposing DC fields could be applied. The filtering capabilities are determined by the applied DC gradient and the TW parameters, such as speed, amplitude, and the TW sequence (i.e., the duty cycle of the traveling wave). The effects of different parameters on the sensitivity and the ion mobility (IM) resolution of the device have been investigated. By appropriately choosing the DC gradient and TW parameters for the two sections, it is possible to transmit ions of a selected mobility while filtering out others of both higher and lower mobility. The novel device described here provides a basis for the targeted analysis of compounds based upon the continuous selection of ions according to their mobility and without the need for high electric fields or pulsed injection. Graphical abstract ᅟ.
在此，我们探索在单个设备中应用恒定场和振荡场的组合，以基于离子迁移率实现离子的连续分离和过滤。所研究的设备允许在无损离子操纵（SLIM）模块的结构中利用射频（rf）、直流（DC）场和行波（TW）的组合来限制和操纵离子。我们已经从理论和实验上研究了一种基于离子迁移率的离子连续过滤概念，其中离子通过两个区域进行迁移率分离和选择，这两个区域都结合了行波和恒定场，对离子提供相反的力。SLIM模块由具有镜像电极阵列的两个表面组成，并有两个可以施加不同行波和相反直流场的区域。过滤能力由所施加的直流梯度和行波参数决定，例如速度、幅度和行波序列（即行波的占空比）。已经研究了不同参数对该设备灵敏度和离子迁移率（IM）分辨率的影响。通过为两个部分适当选择直流梯度和行波参数，可以传输选定迁移率的离子，同时过滤掉其他迁移率更高和更低的离子。本文所述的新型设备为基于根据离子迁移率连续选择离子且无需高电场或脉冲注入的化合物靶向分析提供了基础。图形摘要ᅟ。
