Wang Guanghua, Xu Shuailiang, Su Hongfei, Chen Biao, Huang Wen, Liang Jiayuan, Wang Yinghui, Yu Kefu
1Guangxi Key Laboratory on the Study of Coral Reefs in the South China Sea, Nanning 530004, PR China.
2Coral Reef Research Center of China, Guangxi University, Nanning 530004, PR China.
Int J Syst Evol Microbiol. 2018 Oct;68(10):3292-3295. doi: 10.1099/ijsem.0.002984. Epub 2018 Aug 17.
A Gram-stain-negative, non-spore-forming, aerobic, motile, ovoid or rod-shaped bacterium, designated strain C34, was isolated from a Porites species coral on Weizhou Island, China. Optimal growth occurred in 4 % NaCl (w/v), at 30 °C and pH 8. The only detected respiratory quinone was Q-8. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, two unidentified ninhydrin-positive lipids, one ninhydrin-positive unidentified phospholipid and two unidentified polar lipids. The genome DNA G+C content was 56.7 mol%. The major cellular fatty acids were C16 : 1ω7c/iso-C15 : 0 2-OH, C16 : 0, C18 : 1ω7c/ω6c, C18 : 0 and iso-C11 : 0 3-OH. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain C34 forms a stable cluster with Motiliproteus sediminis CICC 10858 (with the highest sequence similarity of 95.7 %). Strain C34 was also physiologically and chemical taxonomically similar to M. sediminis CICC 10858, although they could be distinguished by colony colour on 2216E agar, the flagellum position and the diphosphatidylglycerol content in the cellular polar lipid. Thus, strain C34 is suggested to represent a new species in the genus Motiliproteus, for which the name Motiliproteus coralliicola is proposed. The type strain is C34 (=MCCC 1K03462=KCTC 62319).
从中国涠洲岛的一种鹿角珊瑚中分离出一株革兰氏染色阴性、不产芽孢、需氧、运动、卵形或杆状的细菌，命名为菌株C34。在4%（w/v）NaCl、30°C和pH 8条件下生长最佳。唯一检测到的呼吸醌是Q-8。主要极性脂为磷脂酰乙醇胺、磷脂酰甘油、二磷脂酰甘油、两种未鉴定的茚三酮阳性脂、一种茚三酮阳性未鉴定磷脂和两种未鉴定的极性脂。基因组DNA G+C含量为56.7 mol%。主要细胞脂肪酸为C16:1ω7c/异-C15:0 2-OH、C16:0、C18:1ω7c/ω6c、C18:0和异-C11:0 3-OH。基于16S rRNA基因序列的系统发育分析表明，菌株C34与沉积物动变形菌CICC 10858形成一个稳定的聚类（序列相似性最高为95.7%）。菌株C34在生理和化学分类上也与沉积物动变形菌CICC 10858相似，尽管它们可以通过在2216E琼脂上的菌落颜色、鞭毛位置和细胞极性脂中二磷脂酰甘油的含量来区分。因此，建议菌株C34代表动变形菌属中的一个新物种，为此提出名称珊瑚动变形菌。模式菌株为C34（=MCCC 1K03462=KCTC 62319）。
