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基于染料的线粒体温度测定法及其在棕色脂肪细胞产热中的应用。

Dye-based mito-thermometry and its application in thermogenesis of brown adipocytes.

作者信息

Xie Tao-Rong, Liu Chun-Feng, Kang Jian-Sheng

机构信息

CAS Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, 200031 China.

出版信息

Biophys Rep. 2017;3(4):85-91. doi: 10.1007/s41048-017-0039-6. Epub 2017 May 13.

DOI:10.1007/s41048-017-0039-6
PMID:29238745
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5719795/
Abstract

UNLABELLED

Mitochondrion is the main intracellular site for thermogenesis and attractive energy expenditure targeting for obesity therapy. Here, we develop a method of mitochondrial thermometry based on Rhodamine B methyl ester, which equilibrates as a thermosensitive mixture of nonfluorescent and fluorescent resonance forms. Using this approach, we are able to demonstrate that the efficacy of norepinephrine-induced thermogenesis is low, and measure the maximum transient rate of temperature increase in brown adipocytes.

ELECTRONIC SUPPLEMENTARY MATERIAL

The online version of this article (doi:10.1007/s41048-017-0039-6) contains supplementary material, which is available to authorized users.

摘要

未标注

线粒体是细胞内主要的产热场所，也是肥胖治疗中极具吸引力的能量消耗靶点。在此，我们开发了一种基于罗丹明B甲酯的线粒体温度测定方法，该物质以非荧光和荧光共振形式的热敏混合物达到平衡。使用这种方法，我们能够证明去甲肾上腺素诱导的产热效果较低，并测量棕色脂肪细胞中温度升高的最大瞬态速率。

电子补充材料

本文的在线版本（doi:10.1007/s41048-017-0039-6）包含补充材料，授权用户可获取。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1d6/5719795/20a271a665e7/41048_2017_39_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1d6/5719795/3746e42fd776/41048_2017_39_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1d6/5719795/2f5605b3c3b4/41048_2017_39_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1d6/5719795/75fb0e0eeb9e/41048_2017_39_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1d6/5719795/20a271a665e7/41048_2017_39_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1d6/5719795/3746e42fd776/41048_2017_39_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1d6/5719795/2f5605b3c3b4/41048_2017_39_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1d6/5719795/75fb0e0eeb9e/41048_2017_39_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1d6/5719795/20a271a665e7/41048_2017_39_Fig4_HTML.jpg

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