一种用于可视化活细胞中线粒体温度的比率荧光分子探针。
A ratiometric fluorescent molecular probe for visualization of mitochondrial temperature in living cells.
作者信息
Homma Mitsumasa, Takei Yoshiaki, Murata Atsushi, Inoue Takafumi, Takeoka Shinji
机构信息
Department of Life Science & Medical Bioscience, Graduate School of Advanced Science & Engineering, Waseda University, TWins 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan.
出版信息
Chem Commun (Camb). 2015 Apr 11;51(28):6194-7. doi: 10.1039/c4cc10349a.
Mitochondrial thermodynamics is the key to understand cellular activities related to homeostasis and energy balance. Here, we report the first ratiometric fluorescent molecular probe (Mito-RTP) that is selectively localized in the mitochondria and visualize the temperature. We confirmed that Mito-RTP could work as a ratiometric thermometer in a cuvette and living cells.
线粒体热力学是理解与体内平衡和能量平衡相关的细胞活动的关键。在此，我们报告了首个选择性定位于线粒体并能可视化温度的比率荧光分子探针（Mito-RTP）。我们证实Mito-RTP可作为比色温度计在比色皿和活细胞中发挥作用。
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