Simione Frank, Sharp Tim
ATCC, 10801 University Blvd, Manassas, VA, 20110, USA.
ICON, Plc. 123 Smith Street, Farmingdale, NY, 11735, USA.
In Vitro Cell Dev Biol Anim. 2017 Dec;53(10):888-895. doi: 10.1007/s11626-017-0214-6. Epub 2017 Dec 13.
Successfully recovering stored cryopreserved cells requires attention to two major factors, i.e., storage temperature and proper handling of the frozen samples. These key considerations are in turn impacted by how the inventory system is constructed, the frequency and process for retrieving specimens, as well as how the material is secured. In addition, shipping biological materials requires attention to the type of material transported, adherence to regulatory requirements, packaging materials and proper assembly, labeling, and engaging reputable carriers. This paper provides an overview discussion of the current best practices for storing, handling, and shipping of cryopreserved cells and provides references to more detailed information on specific topics.
成功复苏储存的冷冻保存细胞需要关注两个主要因素，即储存温度和对冷冻样本的妥善处理。这些关键因素又会受到库存系统的构建方式、检索标本的频率和流程以及材料的固定方式的影响。此外，运输生物材料需要关注所运输材料的类型、遵守监管要求、包装材料及正确组装、标签以及选择信誉良好的运输商。本文概述了冷冻保存细胞的储存、处理和运输的当前最佳实践，并提供了特定主题更详细信息的参考文献。
