Til David, Amaral Vera L L, Salvador Rafael A, Senn Alfred, Paula Thais S de
Vale do Itajaí University - UNIVALI - Itajaí/SC, Brazil.
F.A.B.E.R Foundation, Lausanne, Switzerland - Lausanne, Switzerland.
JBRA Assist Reprod. 2016 Dec 1;20(4):217-221. doi: 10.5935/1518-0557.20160042.
This study aimed to test the effects on sperm viability of transporting cryopreserved semen samples on dry ice.
Twenty normozoospermic semen samples were cryopreserved and divided into five groups. The samples in Group 1 were immersed in liquid nitrogen throughout the experiment in cryogenic storage tanks; the cryopreserved straws in Group 2 were placed in a Styrofoam box containing dry ice and kept under these conditions for 48 hours; the samples in Group 3 were kept for 48 hours on dry ice under the same conditions as the Group 2 samples, and were then moved to a storage tank filled with liquid nitrogen; Group 4 samples were also kept for 48 hours in dry ice storage, and the Styrofoam box containing the samples was shipped by plane to assess the effects of shipping; the samples in Group 5 were shipped together with the Group 4 samples and were placed in a storage tank with liquid nitrogen after spending 48 hours stored on dry ice. After thawing, sperm parameters were analyzed for viability, vitality, and motility; spermatozoa were also tested for mitochondrial activity.
Significant decreases in motility recovery rates (P=0.01) and vitality (P=0.001) were observed in all groups when compared to the control group. Mitochondrial activity was significantly decreased only in Group 5 (P=0.04), as evidenced by greater numbers of sperm cells not stained by reagent 3,3'-diaminobenzidine.
Transportation did not affect the quality of cryopreserved semen samples, but dry ice as a means to preserve the samples during transportation had detrimental effects upon the sperm parameters assessed in this study.
本研究旨在测试用干冰运输冷冻保存的精液样本对精子活力的影响。
将20份正常精子精液样本冷冻保存并分为五组。第1组样本在整个实验过程中浸没于低温储存罐的液氮中；第2组冷冻吸管置于装有干冰的泡沫箱中，并在这些条件下保存48小时；第3组样本在与第2组样本相同的条件下在干冰上保存48小时，然后转移至装满液氮的储存罐中；第4组样本也在干冰储存中保存48小时，装有样本的泡沫箱通过飞机运输以评估运输的影响；第5组样本与第4组样本一起运输，并在干冰上保存48小时后置于装有液氮的储存罐中。解冻后，分析精子参数的活力、生命力和运动能力；还对精子进行线粒体活性测试。
与对照组相比，所有组的运动恢复率（P = 0.01）和生命力（P = 0.001）均显著降低。仅第5组线粒体活性显著降低（P = 0.04），这由更多未被3,3'-二氨基联苯胺试剂染色的精子细胞所证明。
运输不影响冷冻保存的精液样本质量，但干冰作为运输过程中保存样本的手段对本研究评估的精子参数有不利影响。
