de Oliveira Louisi Souza, Tschoeke Diogo Antonio, Magalhães Lopes Ana Carolina Rubem, Sudatti Daniela Bueno, Meirelles Pedro Milet, Thompson Cristiane C, Pereira Renato Crespo, Thompson Fabiano L
Instituto de Biologia, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil.
Núcleo em Ecologia e Desenvolvimento Sócio-Ambiental de Macaé (NUPEM), Universidade Federal do Rio de Janeiro, Macaé, Rio de Janeiro, Brazil.
mSphere. 2017 Dec 6;2(6). doi: 10.1128/mSphere.00094-17. eCollection 2017 Nov-Dec.
The ability to recognize and respond to the presence of microbes is an essential strategy for seaweeds to survive in the marine environment, but understanding of molecular seaweed-microbe interactions is limited. clones were inoculated with the marine bacterium . The seaweed RNA was sequenced, providing an unprecedentedly high coverage of the transcriptome of , and the gene expression levels were compared between control and inoculated samples after 24, 48, and 72 h. Transcriptomic changes in in the presence of include the upregulation of genes that participate in signaling pathways described here for the first time as a response of seaweeds to microbes. Genes coding for defense-related transcription activators, reactive oxygen species metabolism, terpene biosynthesis, and energy conversion pathways were upregulated in inoculated samples of , indicating an integrated defensive system in seaweeds. This report contributes significantly to the current knowledge about the molecular mechanisms involved in the highly dynamic seaweed-bacterium interactions. Marine bacteria are part of the healthy microbiota associated with seaweeds, but some species, such as spp., are frequently associated with disease outbreaks, especially in economically valuable cultures. In this context, the ability of seaweeds to recognize microbes and, when necessary, activate defense mechanisms is essential for their survival. However, studies dedicated to understanding the molecular components of the immune response in seaweeds are rare and restricted to indirect stimulus. This work provides an unprecedentedly large-scale evaluation of the transcriptional changes involved in microbe recognition, cellular signaling, and defense in the red seaweed in response to the marine bacterium . By expanding knowledge about seaweed-bacterium interactions and about the integrated defensive system in seaweeds, this work offers the basis for the development of tools to increase the resistance of cultured seaweeds to bacterial infections.
识别和应对微生物的存在是海藻在海洋环境中生存的一项基本策略，但对海藻与微生物分子相互作用的了解有限。将克隆体接种了海洋细菌。对海藻RNA进行测序，提供了前所未有的高转录组覆盖率，并且比较了对照样本和接种样本在24、48和72小时后的基因表达水平。在存在[具体细菌名称未给出]的情况下，[海藻名称未给出]的转录组变化包括首次作为海藻对微生物的反应而在此描述的参与信号通路的基因上调。编码防御相关转录激活因子、活性氧代谢、萜类生物合成和能量转换途径的基因在[海藻名称未给出]的接种样本中上调，表明海藻中存在一个综合防御系统。本报告对当前关于高度动态的海藻 - 细菌相互作用所涉及的分子机制的认识有重大贡献。海洋细菌是与海藻相关的健康微生物群的一部分，但一些物种，如[具体细菌名称未给出]属，经常与疾病爆发有关，特别是在具有经济价值的养殖中。在这种情况下，海藻识别微生物并在必要时激活防御机制的能力对其生存至关重要。然而，致力于了解海藻免疫反应分子成分的研究很少，且仅限于间接刺激。这项工作对红海藻[海藻名称未给出]响应海洋细菌[具体细菌名称未给出]时涉及微生物识别、细胞信号传导和防御的转录变化进行了前所未有的大规模评估。通过扩展关于海藻 - 细菌相互作用以及海藻中综合防御系统的知识，这项工作为开发提高养殖海藻对细菌感染抗性的工具提供了基础。
