Diretoria de Pesquisas, Instituto de Pesquisas Jardim Botânico do Rio de Janeiro, Rio de Janeiro 22460-030, Brazil.
Departamento de Biotecnologia Marinha, Instituto de Estudos do Mar Almirante Paulo Moreira-IEAPM, Arraial do Cabo 28930-000, RJ, Brazil.
Cells. 2023 Sep 12;12(18):2259. doi: 10.3390/cells12182259.
seaweed species synthesize a broad range of secondary metabolites, mainly terpenes (e.g., elatol), exhibiting diverse ecological roles, such as defense against fouling and herbivores. Recently, an intricate cellular machinery was described concerning terpenes biosynthetic pathways, storage inside (CC), and regulated exocytosis in these species. But for seaweeds in general, the proteins involved in transmembrane transport of secondary metabolites remain unknown. Assays with Rhodamine-123 and cyclosporine A (CSA) revealed the presence of ABC transporters in CC membrane of . In vivo incubation assays with CSA resulted in CC morphological changes, reduced intracellular elatol concentrations, and increased biofouling cover on the seaweed surface. Cultivation assays in the presence of a marine pathogenic bacteria induced the expression of ABC proteins belonging to the subfamilies ABCB, ABCD, ABCF, and ABCG. The latter subfamily is known to be associated with the transport of plant terpenes. Our results shed new light on the role of ABC proteins in key mechanisms of the defensive system in seaweeds against fouling and herbivory.
海藻物种合成广泛的次生代谢物，主要是萜类化合物（例如，艾洛醇），具有多种生态作用，如防止污损和草食动物的侵害。最近，描述了涉及萜类生物合成途径、在 （CC） 中的储存和这些物种中受调控的胞吐作用的复杂细胞机制。但对于一般的海藻来说，参与次生代谢物跨膜运输的蛋白质仍然未知。用 Rhodamine-123 和环孢菌素 A（CSA）进行的测定表明，CC 膜中存在 ABC 转运蛋白。用 CSA 进行的体内孵育测定导致 CC 形态发生变化，细胞内艾洛醇浓度降低，海藻表面的生物污损覆盖物增加。在存在海洋致病性细菌的培养测定中，诱导了属于 ABCB、ABCD、ABCF 和 ABCG 亚家族的 ABC 蛋白的表达。后一个亚家族与植物萜类化合物的运输有关。我们的结果为 ABC 蛋白在海藻防御系统中防止污损和草食动物侵害的关键机制中的作用提供了新的认识。
