Sakulsathaporn Arpakorn, Wonnapinij Passorn, Vuttipongchaikij Supachai, Apisitwanich Somsak
Center for Agricultural Biotechnology, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom, 73140, Thailand.
Center of Excellence on Agricultural Biotechnology: (AG-BIO/PERDO-CHE), Kasetsart University, 50 Ngarm Wong Wan Road, Chattuchak, Bangkok, 10900, Thailand.
BMC Res Notes. 2017 Dec 16;10(1):740. doi: 10.1186/s13104-017-3077-8.
Borassus flabellifer or Asian Palmyra palm is widely distributed in South and Southeast Asia and is horticultural and economic importance for its fruit and palm sugar production. However, its population is in rapid decline, and only a few genetic data are available. We sequenced the complete chloroplast (cp) genome of B. flabellifer to provide its genetic data for further utilization.
The cp genome was obtained by Illumina sequencing and manual gap fillings providing 160,021 bp in length containing a pair of inverted repeats (IRs) with 27,256 bp. These IRs divide the genome into a large single copy region 87,444 bp and a small single copy region 18,065 bp. In total, 113 unique genes, 134 SSRs and 47 large repeats were identified. This is the first complete cp genome reported in the genus Borassus. A comparative analysis among members of the Borasseae tribe revealed that the B. flabellifer cp genome is, so far, the largest and the cp genomes of this tribe have a similar structure, gene number and gene arrangement. A phylogenetic tree reconstructed based on 74 protein-coding genes from 70 monocots demonstrates short branch lengths indicating slow evolutionary rates of cp genomes in family Arecaceae.
扇叶糖棕广泛分布于南亚和东南亚，因其果实和棕榈糖生产具有园艺和经济重要性。然而，其种群数量正在迅速下降，且仅有少量遗传数据可用。我们对扇叶糖棕的完整叶绿体（cp）基因组进行了测序，以提供其遗传数据用于进一步利用。
通过Illumina测序和人工填补缺口获得了cp基因组，其长度为160,021 bp，包含一对长度为27,256 bp的反向重复序列（IRs）。这些IRs将基因组分为一个87,444 bp的大单拷贝区域和一个18,065 bp的小单拷贝区域。总共鉴定出113个独特基因、134个简单序列重复（SSRs）和47个大重复序列。这是扇叶糖棕属中报道的首个完整cp基因组。对棕榈科部落成员的比较分析表明，到目前为止，扇叶糖棕的cp基因组是最大的，且该部落的cp基因组具有相似的结构、基因数量和基因排列。基于70种单子叶植物的74个蛋白质编码基因重建的系统发育树显示分支长度较短，表明棕榈科cp基因组的进化速率较慢。
