Varadarajan Ramya, Hammer John A, Rusan Nasser M
From the Cell Biology and Physiology Center, NHLBI, National Institutes of Health, Bethesda Maryland 20892.
From the Cell Biology and Physiology Center, NHLBI, National Institutes of Health, Bethesda Maryland 20892
J Biol Chem. 2017 Dec 15;292(50):20410-20411. doi: 10.1074/jbc.H117.806018.
The scaffolding protein AKAP350A is known to localize to the centrosome and the Golgi, but the molecular details of its function at the centrosome remain elusive. Using structure-function analyses, protein interaction assays, and super-resolution microscopy, Kolobova now identify AKAP350A's specific location and protein partners at the centrosome. The authors further define an autoregulatory mechanism that likely controls AKAP350A's ability to nucleate microtubule growth.
支架蛋白AKAP350A已知定位于中心体和高尔基体,但它在中心体发挥功能的分子细节仍不清楚。通过结构-功能分析、蛋白质相互作用测定和超分辨率显微镜技术,科洛博娃等人现在确定了AKAP350A在中心体的具体位置和蛋白质相互作用伙伴。作者进一步定义了一种可能控制AKAP350A促使微管生长能力的自动调节机制。