Ahamed Maqusood, Khan M A Majeed, Akhtar Mohd Javed, Alhadlaq Hisham A, Alshamsan Aws
King Abdullah Institute for Nanotechnology, King Saud University, Riyadh, Saudi Arabia.
Department of Physics and Astronomy, College of Science, King Saud University, Riyadh, Saudi Arabia.
Sci Rep. 2017 Dec 15;7(1):17662. doi: 10.1038/s41598-017-17559-9.
We investigated the anticancer potential of Ag-doped (0.5-5%) anatase TiO NPs. Characterization study showed that dopant Ag was well-distributed on the surface of host TiO NPs. Size (15 nm to 9 nm) and band gap energy (3.32 eV to 3.15 eV) of TiO NPs were decreases with increasing the concentration of Ag dopant. Biological studies demonstrated that Ag-doped TiO NP-induced cytotoxicity and apoptosis in human liver cancer (HepG2) cells. The toxic intensity of TiO NPs was increases with increasing the amount of Ag-doping. The Ag-doped TiO NPs further found to provoke reactive oxygen species (ROS) generation and antioxidants depletion. Toxicity induced by Ag-doped TiO NPs in HepG2 cells was efficiently abrogated by antioxidant N-acetyl-cysteine (ROS scavenger). We also found that Ag-doped TiO NPs induced cytotoxicity and oxidative stress in human lung (A549) and breast (MCF-7) cancer cells. Interestingly, Ag-doped TiO NPs did not cause much toxicity to normal cells such as primary rat hepatocytes and human lung fibroblasts. Overall, we found that Ag-doped TiO NPs have potential to selectively kill cancer cells while sparing normal cells. This study warranted further research on anticancer potential of Ag-doped TiO NPs in various types of cancer cells and in vivo models.
我们研究了银掺杂（0.5 - 5%）的锐钛矿型二氧化钛纳米颗粒的抗癌潜力。表征研究表明，掺杂剂银在主体二氧化钛纳米颗粒表面分布良好。随着银掺杂浓度的增加，二氧化钛纳米颗粒的尺寸（从15纳米降至9纳米）和带隙能量（从3.32电子伏特降至3.15电子伏特）降低。生物学研究表明，银掺杂的二氧化钛纳米颗粒可诱导人肝癌（HepG2）细胞产生细胞毒性和凋亡。二氧化钛纳米颗粒的毒性强度随着银掺杂量的增加而增加。进一步发现，银掺杂的二氧化钛纳米颗粒可引发活性氧（ROS）生成并消耗抗氧化剂。抗氧化剂N - 乙酰半胱氨酸（ROS清除剂）可有效消除银掺杂的二氧化钛纳米颗粒在HepG2细胞中诱导的毒性。我们还发现，银掺杂的二氧化钛纳米颗粒可诱导人肺癌（A549）和乳腺癌（MCF - 7）细胞产生细胞毒性和氧化应激。有趣的是，银掺杂的二氧化钛纳米颗粒对原代大鼠肝细胞和人肺成纤维细胞等正常细胞没有造成太大毒性。总体而言，我们发现银掺杂的二氧化钛纳米颗粒有潜力选择性地杀死癌细胞，同时 sparing normal cells（此处英文有误，推测应为“保护正常细胞”）。这项研究有必要进一步研究银掺杂的二氧化钛纳米颗粒在各种类型癌细胞和体内模型中的抗癌潜力。
