Kiper Ilkser Erdem, Bloomer Paulette, Borsa Philippe, Hoareau Thierry Bernard
Molecular Ecology and Evolution Programme, Department of Genetics, University of Pretoria, Private Bag X20, Hatfield, Pretoria, 0028, South Africa.
UMR 250 "Ecologie marine tropicale des océans Pacifique et Indien", Institut de recherche pour le développement (IRD), BP A5, 98848, Nouméa, New Caledonia.
Mol Biol Rep. 2018 Feb;45(1):19-25. doi: 10.1007/s11033-017-4136-x. Epub 2017 Dec 15.
Rabbitfishes are reef-associated fishes that support local fisheries throughout the Indo-West Pacific region. Sound management of the resource requires the development of molecular tools for appropriate stock delimitation of the different species in the family. Microsatellite markers were developed for the cordonnier, Siganus sutor, and their potential for cross-amplification was investigated in 12 congeneric species. A library of 792 repeat-containing sequences was built. Nineteen sets of newly developed primers, and 14 universal finfish microsatellites were tested in S. sutor. Amplification success of the 19 Siganus-specific markers ranged from 32 to 79% in the 12 other Siganus species, slightly decreasing when the genetic distance of the target species to S. sutor increased. Seventeen of these markers were polymorphic in S. sutor and were further assayed in S. luridus, S. rivulatus, and S. spinus, of which respectively 9, 10 and 8 were polymorphic. Statistical power analysis and an analysis of molecular variance showed that subtle genetic differentiation can be detected using these markers, highlighting their utility for the study of genetic diversity and population genetic structure in rabbitfishes.
狐篮子鱼是与珊瑚礁相关的鱼类，在整个印度-西太平洋地区为当地渔业提供支持。对该资源进行合理管理需要开发分子工具，以便对该科不同物种进行适当的种群划分。为细纹狐篮子鱼（Siganus sutor）开发了微卫星标记，并在12个同属物种中研究了其交叉扩增的潜力。构建了一个包含792个含重复序列的文库。在细纹狐篮子鱼中测试了19组新开发的引物和14个通用的硬骨鱼微卫星。19个狐篮子鱼特异性标记在其他12个狐篮子鱼物种中的扩增成功率在32%至79%之间，当目标物种与细纹狐篮子鱼的遗传距离增加时，扩增成功率略有下降。其中17个标记在细纹狐篮子鱼中具有多态性，并在黄斑狐篮子鱼（S. luridus）、湾口狐篮子鱼（S. rivulatus）和尖吻狐篮子鱼（S. spinus）中进一步检测，其中分别有9个、10个和8个具有多态性。统计功效分析和分子方差分析表明，使用这些标记可以检测到细微的遗传分化，突出了它们在狐篮子鱼遗传多样性和种群遗传结构研究中的实用性。
