基于适配体和银染纳米金属复合材料的痕量铅离子的可视化检测。
Visual detection of trace lead ion based on aptamer and silver staining nano-metal composite.
机构信息
Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics - Hubei Bioinformatics & Molecular Imaging Key Laboratory, Key Laboratory of Biomedical Photonics (HUST, Ministry of Education), Collaborative Innovation Center for Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, PR China.
Key Laboratory of Optoelectronic Chemical Materials and Devices, Ministry of Education, Jianghan University, Wuhan 430056, PR China.
出版信息
Colloids Surf B Biointerfaces. 2018 Feb 1;162:415-419. doi: 10.1016/j.colsurfb.2017.12.011. Epub 2017 Dec 11.
In this paper, visual detection of trace lead ion was established by aptamer and silver staining. The basic strategy was that aminated PS2.M aptamer was immobilized onto slide and formed stable G-quadruplex structure. PbS was generated by adding S, and it catalyzed subsequent silver staining reaction, through the silver staining amplification effect, the slide presented visible ash black. The gray value of slide after silver staining was analyzed and the semi-quantitative detection of Pb in solution was realized. The results showed that optical darkness ratio (ODR) and logarithmic value of Pb concentration had a good linear relationship (R = 0.951) over the range of 0.5-10 μM. In addition, there was no obvious interference of other common metal ions for the detection, indicating that this method presented outstanding selectivity. And it was also used for qualitative and semi-quantitative determination of Pb in soil sample successfully.
本文建立了一种基于适配体和银染的痕量铅离子可视化检测方法。基本策略是将氨基化 PS2.M 适配体固定在载玻片上，并形成稳定的 G-四链体结构。加入 S 后生成 PbS，并催化随后的银染反应，通过银染的放大效应，载玻片呈现出明显的灰黑色。对银染后的载玻片进行灰度值分析，实现了溶液中 Pb 的半定量检测。结果表明，在 0.5-10 μM 范围内，光学暗度比 (ODR) 与 Pb 浓度的对数呈良好的线性关系 (R=0.951)。此外，该检测方法对其他常见金属离子没有明显的干扰，表明该方法具有突出的选择性。并成功用于土壤样品中 Pb 的定性和半定量测定。
Zotero 插件