Department of Chemistry, Isfahan University of Technology, Isfahan 84156-83111, Iran.
Department of Chemistry, Isfahan University of Technology, Isfahan 84156-83111, Iran.
Spectrochim Acta A Mol Biomol Spectrosc. 2019 Apr 15;213:1-5. doi: 10.1016/j.saa.2019.01.036. Epub 2019 Jan 16.
A new and straightforward optical sensor for the colorimetric determination of adenosine (AD) in human urine samples was developed. The sensor comprised silver nanoparticles (AgNPs) as colorimetric elements and anti-AD aptamer (AP) as a recognition probe. In a solution containing AD and high concentration of NaCl, due to the unique binding of AD with AP, the aggregated metal nanomaterials dispersed in the solution, and the color intensity of the solution was changed accordingly. The absorbance of the solution was monitored for AD quantification. The method was applicable for the determination of AD in the concentration range of 60-280 nM with the detection limit of 21 nM. The relative standard deviation ranged from 4.8 to 8.8% for six replicates. The method showed excellent selectivity toward AD checked over some probable interfering compounds. To investigate the performance of AgNPs, the analytical characteristics of the method including linear range, detection limit, selectivity, and precision were compared with those obtained by a common AuNPs-based aptasensor. The reliability of the method was further ascertained for the detection of AD in urine samples of two lung cancer patients with percentage recoveries in the range of 98-107%.
一种用于人尿样中腺苷（AD）比色测定的新型简便光学传感器已经开发出来。该传感器由银纳米粒子（AgNPs）作为比色元件和抗 AD 适体（AP）作为识别探针组成。在含有 AD 和高浓度 NaCl 的溶液中，由于 AD 与 AP 的独特结合，聚集的金属纳米材料在溶液中分散，溶液的颜色强度也相应改变。通过监测溶液的吸光度来定量 AD。该方法适用于在 60-280 nM 的浓度范围内测定 AD，检测限为 21 nM。六个重复的相对标准偏差范围为 4.8%至 8.8%。该方法对 AD 表现出优异的选择性，经检查，对一些可能的干扰化合物具有良好的选择性。为了研究 AgNPs 的性能，将该方法的分析特性，包括线性范围、检测限、选择性和精密度，与常见的基于金纳米粒子（AuNPs）的适体传感器获得的特性进行了比较。该方法在对两名肺癌患者尿液样本中 AD 的检测中的可靠性也得到了进一步证实，回收率在 98%-107%的范围内。
