Institute of Biomedical Sciences, College of Health Sciences, Mekelle University, Ethiopia; Department of Biotechnology, Faculty of Science and Humanities, SRM University, Kattankulathur, 603203, India.
Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Malaysia.
Microb Pathog. 2018 Feb;115:57-63. doi: 10.1016/j.micpath.2017.12.039. Epub 2017 Dec 14.
Zinc oxide nanoparticles (ZnONPs) exhibit abundant biomedical applications. Anisotropic ZnONPs with a defined shape and size were synthesized using Bacillus megaterium (NCIM 2326) cell free extract as a bio-reductant. The study investigated the multidimensional effect of ZnONPs on Helicobacter pylori strains and assessed its biosafety in normal human mesenchymal stem cells (hMSc). The highly stable ZnONPs were produced using B. megaterium and Zinc nitrate as a precursor. The phase of ZnONPs formation and structural characterization were performed by UV- visible (UV-Vis), Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) and Field Emission Scanning electron microscopy (FESEM) analysis. Furthermore, the ZnONPs exhibited higher biocompatibility against human mesenchymal stem cells (hMSC) and proved to be potentially safe in mammalian cells. Corroborating the current investigation, we described the anti-H. Pylori dosage of ZnONPs was safe to hMSC and could efficiently use as nano-antibiotic.
氧化锌纳米粒子（ZnONPs）在生物医学领域有广泛的应用。本研究采用巨大芽孢杆菌（NCIM 2326）无细胞提取物作为生物还原剂，成功合成了具有特定形状和尺寸的各向异性 ZnONPs。该研究探讨了 ZnONPs 对幽门螺杆菌菌株的多维影响，并评估了其在正常人类间充质干细胞（hMSc）中的生物安全性。使用巨大芽孢杆菌和硝酸锌作为前体，制备了高度稳定的 ZnONPs。通过紫外-可见分光光度计（UV-Vis）、傅里叶变换红外光谱（FTIR）、X 射线衍射（XRD）和场发射扫描电子显微镜（FESEM）分析，对 ZnONPs 的形成和结构特征进行了研究。此外，ZnONPs 对人骨髓间充质干细胞（hMSC）表现出更高的生物相容性，并被证明在哺乳动物细胞中具有潜在的安全性。与目前的研究一致，我们描述了 ZnONPs 的抗 H. Pylori 剂量对 hMSC 是安全的，并可作为有效的纳米抗生素使用。
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