Jabeen Amina, Khan Abdulhameed, Ahmad Pervaiz, Khalid Awais, Ibrahim Wizrah Maha Saeed, Anjum Zeeshan, Alotibi Satam, Aloufi Bandar Hamad, Alanazi Abdulaziz M, Jefri Ohoud A, Ismail Mohamed A
Department of Biotechnology, University of Azad Jammu and Kashmir, Muzaffarabad, 13100, Pakistan.
Department of Physics, University of Azad Jammu and Kashmir, Muzaffarabad, 13100, Pakistan.
Heliyon. 2024 Feb 28;10(6):e27018. doi: 10.1016/j.heliyon.2024.e27018. eCollection 2024 Mar 30.
Despite the success of antibiotics in medicine, the treatment of bacterial infection is still challenging due to emerging resistance and suitable drug delivery system, therefore, innovative approaches focused on nanoparticles based antimicrobial drug delivery systems are highly desired. This research aimed to synthesize () aqueous extract-mediated copper oxide (CuO-Nps) conjugated with levofloxacin (LFX). The synthesized CuO NPs-LFX nano conjugate was confirmed by analysis using scanning electron microscopy (SEM), thermal gravimetric analysis (TGA), and infrared and ultraviolet/visible spectroscopy. Antibacterial activities were assessed in vitro through the agar well diffusion method against six bacterial strains of clinical relevance. CuO NPs confirmed by UV-Vis analysis absorption peak observed at 380 nm. TGA analysis showed 8.98% weight loss between the 400-800 temperature range. The functional group's presence was confirmed by FTIR analysis. Spherical shape nanoparticles with an average particle size of 55 nm were recorded by FESEM. Results from agar well diffusion assay showed that CuO NPs-LFX prohibited the development of both gram-positive and gram-negative bacteria at all established concentrations, and the antibacterial propensity was more pronounced as compared to bare CuO NPs, Levofloxacin and aqueous extract alone. The results showed that gram-negative bacteria are more susceptible to CuO NPs-LFX nano conjugate and at 10 μgmL concentration, form a 10.1 mm zone of inhibition (ZOI), whereas gram-positive bacteria on the same concentration form 9.5 mm ZOI. LFX-loaded CuO NPs antibacterial activity was observed higher than plant extract, bare CuO NPs, and standard drug (Levofloxacin). This study provides a novel approach for the fabrication of biogenic CuO NPs with antibacterial drug levofloxacin and their usage as nano antibiotic carriers against pathogenic bacteria, especially antibiotic-resistant microbes.
尽管抗生素在医学上取得了成功，但由于细菌耐药性的出现和合适的药物递送系统，细菌感染的治疗仍然具有挑战性。因此，人们迫切需要专注于基于纳米颗粒的抗菌药物递送系统的创新方法。本研究旨在合成由水提取物介导的与左氧氟沙星（LFX）共轭的氧化铜（CuO-Nps）。通过扫描电子显微镜（SEM）、热重分析（TGA）以及红外和紫外/可见光谱分析对合成的CuO NPs-LFX纳米共轭物进行了确认。通过琼脂孔扩散法对六种具有临床相关性的细菌菌株进行体外抗菌活性评估。紫外可见分析确认CuO NPs在380nm处观察到吸收峰。热重分析表明在400-800℃温度范围内重量损失8.98%。通过傅里叶变换红外光谱（FTIR）分析确认了官能团的存在。场发射扫描电子显微镜（FESEM）记录到平均粒径为55nm的球形纳米颗粒。琼脂孔扩散试验结果表明，在所有设定浓度下，CuO NPs-LFX均能抑制革兰氏阳性菌和革兰氏阴性菌的生长，并且与单独的裸CuO NPs、左氧氟沙星和水提取物相比，其抗菌倾向更为明显。结果表明，革兰氏阴性菌对CuO NPs-LFX纳米共轭物更敏感，在10μg/mL浓度下形成10.1mm的抑菌圈（ZOI），而相同浓度下的革兰氏阳性菌形成9.5mm的抑菌圈。观察到负载LFX的CuO NPs的抗菌活性高于植物提取物、裸CuO NPs和标准药物（左氧氟沙星）。本研究为制备具有抗菌药物左氧氟沙星的生物源性CuO NPs及其作为抗病原菌特别是耐药微生物的纳米抗生素载体的应用提供了一种新方法。
