Spórna-Kucab Aneta, Milo Angelika, Kumorkiewicz Agnieszka, Wybraniec Sławomir
Department of Analytical Chemistry, Institute C-1, Faculty of Chemical Engineering and Technology, Cracow University of Technology, ul. Warszawska 24, Cracow 31-155, Poland.
Department of Analytical Chemistry, Institute C-1, Faculty of Chemical Engineering and Technology, Cracow University of Technology, ul. Warszawska 24, Cracow 31-155, Poland.
J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Jan 15;1073:96-103. doi: 10.1016/j.jchromb.2017.11.028. Epub 2017 Nov 23.
Betacyanins, natural plant pigments exhibiting antioxidant and chemopreventive properties, were extracted from Celosia spicata (Thouars) Spreng. inflorescences and separated by high-speed counter-current chromatography (HSCCC) in two polar solvent systems composed of: TBME - 1-BuOH - ACN - HO (0.7% HFBA, 2:2:1:5, v/v/v/v) (system I) and EtOH - ACN - 1-PrOH - (NH)SO - HO (0.5:0.5:0.5:1.2:1, v/v/v/v/v) (system II). The systems were used in the head-to-tail (system I) and tail-to-head (system II) mode. The flow rate of the mobile phase was 2.0 ml/min and the column rotation speed was 860 rpm. The retention of the stationary phase was 73.5% (system I) and 80.0% (system II). For the identification of separated betacyanins in the crude extract as well as in the HSCCC fractions, LC-DAD-ESI-MS/MS analyses were performed. Depending on the target compounds, each of the systems exhibit meaningfully different selectivity and applicability. For the pairs of amaranthines (1/1') and betanins (2/2'), the best choice is the system II, but the acylated amaranthine pairs (3/3' and 4/4') can be resolved only in the ion-pair system I. For the indication of the most suitable solvent system for Celosia plumosa hort., Celosia cristata L. and Celosia spicata (Thouars) Spreng. species, the profiles of betacyanins in different plant parts were studied.
从鸡冠花（Celosia spicata (Thouars) Spreng.）花序中提取了具有抗氧化和化学预防特性的天然植物色素甜菜色素，并通过高速逆流色谱法（HSCCC）在由以下组成的两种极性溶剂系统中进行分离：叔丁基甲基醚 - 正丁醇 - 乙腈 - 水（0.7% 氢氟酸，2:2:1:5，v/v/v/v）（系统 I）和乙醇 - 乙腈 - 正丙醇 - 硫酸铵 - 水（0.5:0.5:0.5:1.2:1，v/v/v/v/v）（系统 II）。这些系统分别以头对尾（系统 I）和尾对头（系统 II）模式使用。流动相流速为 2.0 ml/min，柱转速为 860 rpm。固定相保留率分别为 73.5%（系统 I）和 80.0%（系统 II）。为了鉴定粗提物以及 HSCCC 馏分中分离出的甜菜色素，进行了液相色谱 - 二极管阵列 - 电喷雾串联质谱（LC-DAD-ESI-MS/MS）分析。根据目标化合物的不同，每个系统表现出明显不同的选择性和适用性。对于苋红素对（1/1'）和甜菜红素对（2/2'），最佳选择是系统 II，但酰化苋红素对（3/3' 和 4/4'）只能在离子对系统 I 中分离。为了确定最适合羽状鸡冠花（Celosia plumosa hort.）、普通鸡冠花（Celosia cristata L.）和鸡冠花（Celosia spicata (Thouars) Spreng.）物种的溶剂系统，研究了不同植物部位的甜菜色素谱。
