Wu Xia, Zhang Feng, Hu Peng, Chen Lumin, Han Guocan, Bai Weixian, Luo Jingfeng, Chen Ran, Zhou Yurong, Sun Jihong, Yang Xiaoming
Department of Radiology, Sir Run Run Shaw Hospital, Zhejiang University College of Medicine and Sir Run Run Shaw Institute of Clinical Medicine of Zhejiang University, Hangzhou, Zhejiang 310016, P.R. China.
Image-Guided Bio-Molecular Intervention Research and Section of Vascular and Interventional Radiology, Department of Radiology, University of Washington School of Medicine, Seattle, WA 98195, USA.
Oncol Lett. 2017 Dec;14(6):7250-7256. doi: 10.3892/ol.2017.7145. Epub 2017 Oct 6.
A novel, minimally invasive interventional technique, radiofrequency heat (RFH), has been suggested to improve the efficacy of chemotherapy for solid organ tumors. However, the treatment for prostate cancer has not been completely characterized. The aim of the present study was to investigate the and efficiency of chemotherapy in combination with RFH for the treatment of prostate cancer. The following four treatment groups were included: i) No treatment (control); ii) RFH-only; iii) chemotherapy (docetaxel)-only; and iv) combination therapy of docetaxel and RFH in human prostate cancer (HPC) cell lines and mice with HPC xenografts. In the experiments, a heating guidewire was attached under the bottom of the last chamber of the four-chamber cell culture slide, and was then connected to a radiofrequency (RF) generator. In the experiments, a tumor model was generated by subcutaneously injecting human prostate cancer cells into 24 male nu/nu mice. RFH was conducted by inserting the 0.022-inch heating-guidewire into the tumor. The follow-up magnetic resonance imaging demonstrated a significant reduction in the average tumor size in animals treated with combination therapy compared with those receiving RFH-only and chemotherapy-only. The number of apoptotic cells and the average apoptotic index of the combination therapy group were significantly higher compared with those of the other three treatment groups. In conclusion, the results of the present study suggested that RFH is able to increase the therapeutic efficiency of docetaxel in prostate cancer, and this study serves as a foundation for the future development of an interventional molecular image-guided local treatment strategy for prostate cancer that integrates RF technology, interventional oncology and direct intratumoral chemotherapy, as a replacement for systemic chemotherapy.
一种新型的微创介入技术——射频热疗（RFH），已被提出用于提高实体器官肿瘤化疗的疗效。然而，前列腺癌的这种治疗方法尚未完全明确。本研究的目的是探讨化疗联合RFH治疗前列腺癌的疗效和效率。本研究纳入了以下四个治疗组：i）不治疗（对照组）；ii）单纯RFH；iii）单纯化疗（多西他赛）；iv）多西他赛与RFH联合治疗，用于人前列腺癌细胞系和人前列腺癌异种移植小鼠。在细胞实验中，将加热导丝连接在四腔细胞培养载玻片最后一个腔室的底部，然后连接到射频（RF）发生器。在动物实验中，通过将人前列腺癌细胞皮下注射到24只雄性裸鼠体内建立肿瘤模型。通过将0.022英寸的加热导丝插入肿瘤进行RFH治疗。后续的磁共振成像显示，与单纯接受RFH和单纯化疗的动物相比，联合治疗的动物平均肿瘤大小显著减小。联合治疗组的凋亡细胞数量和平均凋亡指数显著高于其他三个治疗组。总之，本研究结果表明，RFH能够提高多西他赛治疗前列腺癌的疗效，本研究为未来开发一种介入分子影像引导的前列腺癌局部治疗策略奠定了基础，该策略整合了射频技术、介入肿瘤学和直接瘤内化疗，以替代全身化疗。
