Almeida Nara Ligia Martins, Saldanha Luiz Leonardo, da Silva Rafaela Alves, Pinke Karen Henriette, da Costa Eliane Ferraz, Porto Vinicius Carvalho, Dokkedal Anne Lígia, Lara Vanessa Soares
a Department of Surgery, Stomatology, Pathology and Radiology, Bauru School of Dentistry , University of São Paulo , Bauru , Brazil.
b Department of Botany, Institute of Biosciences , São Paulo State University (UNESP) , Botucatu , Brazil.
Biofouling. 2018 Jan;34(1):62-73. doi: 10.1080/08927014.2017.1407408. Epub 2017 Dec 18.
Candida biofilms adhere to the internal surface of removable dentures, which is an etiological factor in the pathogenesis of denture stomatitis (DS). Adhesive materials are used at the base of maxillary complete dentures to improve their retention and chewing qualities. This article reports the antimicrobial activity of the enriched fractions of Equisetum giganteum and Punica granatum incorporated into a denture adhesive against C. albicans biofilm. The biofilms were induced on the surface of heat-cured acrylic resin specimens that were previously treated with a mixture of adhesive/herb extracts. The antimicrobial activity was evaluated by CFU counts, XTT reduction, and SEM and CLSM analysis. Both herb extracts amplified the anti-biofilm action of the adhesive on the acrylic resin by up to 12 h. Therefore, when these extracts were combined with COREGA®, they played a collaborative and innovative role in biofilm control and can be considered alternatives for temporary use in the treatment and/or prevention of DS.
念珠菌生物膜附着于可摘义齿的内表面，这是义齿性口炎（DS）发病机制中的一个病因。在上颌全口义齿基托处使用黏附材料以改善其固位和咀嚼性能。本文报道了加入到义齿黏附剂中的木贼和石榴的富集成分对白色念珠菌生物膜的抗菌活性。生物膜在预先用黏附剂/草药提取物混合物处理过的热固化丙烯酸树脂标本表面诱导形成。通过菌落形成单位计数、XTT还原以及扫描电子显微镜和共聚焦激光扫描显微镜分析来评估抗菌活性。两种草药提取物在长达12小时内增强了黏附剂对丙烯酸树脂的抗生物膜作用。因此，当这些提取物与COREGA®结合时，它们在生物膜控制中发挥了协同和创新作用，可被视为治疗和/或预防DS的临时替代方法。
