Venante Helena Sandrini, Chappuis-Chocano Ana Paula, Marcillo-Toala Oscar Oswaldo, da Silva Rafaela Alves, da Costa Rodrigo Moreira Bringel, Pordeus Mariana Domingues, Barraviera Benedito, Ferreira Junior Rui Seabra, Lara Vanessa Soares, Neppelenbroek Karin Hermana, Honório Heitor Marques, Porto Vinicius Carvalho
Department of Periodontics and Prosthodontics, Dental School of Bauru, FOB-USP-University of São Paulo, Al. Octávio Pinheiro Brisola, 9-75, Bauru 17012-901, Brazil.
School of Dentistry, Universidad Espíritu Santo, Samborondon, Guayas 09-01-952, Ecuador.
Materials (Basel). 2021 Mar 26;14(7):1618. doi: 10.3390/ma14071618.
The characteristics of the denture base surface, in combination with the oral environment, promote the colonization and development of biofilm, which is the main cause of denture stomatitis. This study evaluated the effectiveness of fibrin biopolymer with digluconate chlorhexidine or alcoholic extract to prevent biofilm. Conventional heat polymerized and pre-polymerized poly(methyl methacrylate) (PMMA) circular specimens (10 × 2 mm) were fabricated ( = 504) and randomly divided into groups: no treatment (control-CT), fibrin biopolymer coating (FB), fibrin biopolymer with (FBPg), or digluconate of chlorhexidine (FBCh) coating. The specimens were inoculated with SC5314 (1 × 10 cells/mL) and incubated for 24, 48, and 72 h. Crystal violet and colony-forming unit assays were used to quantify the total biofilm biomass and biofilm-living cells. A qualitative analysis was performed using confocal laser scanning microscopy. Data obtained are expressed as means and standard deviations and were statistically analyzed using a three-way analysis of variance (α = 0.05). The FBPg and FBCh groups inhibited the growth of biofilm in both PMMA materials analyzed, with FBCh performing better in all periods evaluated ( < 0.0001). The colony forming unit (CFU) assay showed that the FB group favored the biofilm growth at 24 h and 48 h ( < 0.0001), with no differences with CT group at 72 h ( = 0.790). All groups showed an enhancement in biofilm development up to 72 h ( < 0.0001), except the FBCh group ( = 0.100). No statistical differences were found between the PMMA base materials ( > 0.050), except in the FB group ( < 0.0001). Fibrin biopolymer, albeit a scaffold for the growth of when combined with chlorhexidine digluconate or , demonstrated excellent performance as a drug delivery system, preventing and controlling the formation of denture biofilm.
义齿基托表面的特性，与口腔环境相结合，促进了生物膜的定植和发展，而生物膜是义齿性口炎的主要原因。本研究评估了含葡萄糖酸氯己定或酒精提取物的纤维蛋白生物聚合物预防生物膜的有效性。制作了常规热聚合和预聚合的聚甲基丙烯酸甲酯（PMMA）圆形标本（10×2mm）（n = 504），并随机分为几组：不处理（对照-CT）、纤维蛋白生物聚合物涂层（FB）、含[具体物质]的纤维蛋白生物聚合物（FBPg）或葡萄糖酸氯己定（FBCh）涂层。将标本接种白色念珠菌SC53I4（1×10⁸细胞/mL），并孵育24、48和72小时。采用结晶紫和菌落形成单位测定法来量化生物膜总生物量和生物膜活细胞。使用共聚焦激光扫描显微镜进行定性分析。获得的数据以均值和标准差表示，并使用三因素方差分析进行统计分析（α = 0.05）。FBPg组和FBCh组在分析的两种PMMA材料中均抑制了生物膜的生长，在所有评估时间段内FBCh组表现更佳（P < 0.0001）。菌落形成单位（CFU）测定表明，FB组在24小时和48小时促进了生物膜生长（P < 0.0001），在72小时时与CT组无差异（P = 0.790）。除FBCh组外（P = 0.100），所有组在72小时内生物膜发展均增强（P < 0.0001）。除FB组外（P < 0.0001），在PMMA基托材料之间未发现统计学差异（P > 0.050）。纤维蛋白生物聚合物，尽管与葡萄糖酸氯己定或[具体物质]结合时是白色念珠菌生长的支架，但作为药物递送系统表现出优异性能，可预防和控制义齿生物膜的形成。
