Poltegor-Institute, Opencast Mining Institute, Parkowa 25, 51-616 Wrocław, Poland.
Poltegor-Institute, Opencast Mining Institute, Parkowa 25, 51-616 Wrocław, Poland.
Ecotoxicol Environ Saf. 2018 Mar;149:275-283. doi: 10.1016/j.ecoenv.2017.12.008. Epub 2017 Dec 16.
Due to the progressive development of industrial and technological activities, heavy metal contamination is increasing each year and it poses a serious health and environmental risk. Microorganisms are capable of removing heavy metals from a contaminated environment. In this work, 51 microbial strains were isolated from heavy metal contaminated water and soil. The JAW1 strain, identified as Pseudomonas azotoformans, was selected and applied in bioremediation of the specific mixture of metals (Cd, Cu, and Pb) in an aqueous medium. The Box-Behnken design was used to optimize the biosorption process, with three factors: pH, initial metal concentration, concentration of the biosorbent. For the strain P. azotoformans JAW1, the optimal conditions were pH = 6.0, 25mg/L of each metal and 2g/L, following removal levels were achieved: Cd 44,67%; Cu 63,32%; Pb 78,23%. The possible interactions of cell-metal ions were evaluated using FT-IR analysis. The study indicated the presence of groups, which may be responsible for bonding of metal ions. The studies conducted on bioremediation mechanisms indicated that metal accumulation could occur on the cell surface (biosorption) where the amount of adsorbed metals reached: Cd 98,57%, Cu 69,76%, Pb 88,58%. P. azotoformans JAW1 exhibited a potential for application in the bioremediation of mining wastewater with complex metal contaminations.
由于工业和技术活动的不断发展,重金属污染每年都在增加,对健康和环境构成了严重威胁。微生物具有从污染环境中去除重金属的能力。在这项工作中,从重金属污染的水和土壤中分离出了 51 株微生物菌株。JAW1 菌株被鉴定为固氮假单胞菌,被选择并应用于在水介质中生物修复特定的金属混合物(Cd、Cu 和 Pb)。采用 Box-Behnken 设计优化生物吸附过程,三个因素为:pH 值、初始金属浓度、生物吸附剂浓度。对于 P. azotoformans JAW1 菌株,最佳条件为 pH = 6.0、每种金属 25mg/L 和 2g/L,去除率分别为:Cd 44.67%;Cu 63.32%;Pb 78.23%。使用 FT-IR 分析评估了细胞与金属离子的可能相互作用。研究表明,可能存在一些基团,这些基团可能负责与金属离子结合。对生物修复机制的研究表明,金属积累可能发生在细胞表面(生物吸附),其中吸附的金属量达到:Cd 98.57%;Cu 69.76%;Pb 88.58%。P. azotoformans JAW1 表现出在生物修复含有复杂金属污染的采矿废水中的应用潜力。