Yamada Misuzu, Hirabayashi Kenichi, Kawanishi Aya, Hadano Atsuko, Takanashi Yumi, Izumi Hideki, Kawaguchi Yoshiaki, Mine Tetsuya, Nakamura Naoya, Nakagohri Toshio
Department of Surgery, Tokai University School of Medicine, 143 Shimokasuya, Isehara, 259-1193, Kanagawa, Japan.
Department of Pathology, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa, 259-1193, Japan.
Surg Today. 2018 May;48(5):510-516. doi: 10.1007/s00595-017-1618-3. Epub 2017 Dec 18.
Nectin-1 is a cell adhesion molecule that regulates the formation of adherens junctions and tight junctions. We measured the expression of nectin-1 in cancer-associated fibroblasts (CAFs) in patients with pancreatic ductal adenocarcinoma (PDAC).
Nectin-1 expression was measured via immunohistochemistry using tissue microarray blocks constructed from resected PDAC tissue from 258 patients. We screened for associations between nectin-1 expression and clinicopathological parameters. According to the percentage of CAFs stained, expression was classified as negative at ≤ 30% and positive at > 30%.
Nectin-1 expression was confirmed in CAFs from 64 patients (24.8%), and was associated with lymph node metastasis (p = 0.016), advanced Union for International Cancer Control stage (p = 0.016), perineural invasion (p = 0.022), pancreatic head tumors (p = 0.023), and shorter overall survival (p = 0.003). Multivariate analysis revealed that nectin-1 expression in CAFs was an independent prognostic factor (p = 0.038).
Diffuse nectin-1 expression in the CAFs of PDAC patients is associated with invasion, metastasis, and shorter survival.
NECTIN-1是一种细胞粘附分子，可调节黏附连接和紧密连接的形成。我们测量了胰腺导管腺癌（PDAC）患者癌相关成纤维细胞（CAF）中NECTIN-1的表达。
使用从258例患者切除的PDAC组织构建的组织微阵列块，通过免疫组织化学测量NECTIN-1的表达。我们筛选了NECTIN-1表达与临床病理参数之间的关联。根据CAF染色的百分比，表达分类为≤30%为阴性，>30%为阳性。
在64例患者（24.8%）的CAF中证实了NECTIN-1的表达，并且与淋巴结转移（p = 0.016）、国际癌症控制联盟晚期（p = 0.016）、神经周围侵犯（p = 0.022）、胰头肿瘤（p = 0.023）和较短的总生存期（p = 0.003）相关。多变量分析显示，CAF中NECTIN-1的表达是一个独立的预后因素（p = 0.038）。
PDAC患者CAF中NECTIN-1的弥漫性表达与侵袭、转移和较短生存期相关。
