Iida N, Toida T, Kushi Y, Handa S, Fredman P, Svennerholm L, Ishizuka I
Department of Biochemistry, Teikyo University, School of Medicine, Tokyo, Japan.
J Biol Chem. 1989 Apr 5;264(10):5974-80.
A novel sulfated glycosphingolipid containing a sulfated glucosyl residue was isolated from rat kidney and purified to homogeneity by column chromatographies with DEAE-Sephadex and silica beads. By compositional analyses, permethylation studies, one- and two-dimensional proton magnetic resonance spectroscopy, infrared spectroscopy, negative secondary ion mass spectrometry, solvolysis, and immunostaining on thin layer chromatogram, the structure of this glycolipid was proposed to be HSO3-3Glc beta 1-1Cer (where Cer is ceramide). The ceramide portion consisted of 4-D-hydroxysphinganine as the sole long chain base, and the fatty acid consisted of predominantly tetracosanoic acid, deduced from both composition analysis and negative secondary ion mass spectrometry. The yield of glucosyl sulfatide was about 5 nmol/g of tissue, being about three times as much as that of lactosylceramide sulfate.
从大鼠肾脏中分离出一种含有硫酸化葡萄糖残基的新型硫酸化糖鞘脂，并通过DEAE-葡聚糖和硅胶柱色谱法将其纯化至同质。通过组成分析、全甲基化研究、一维和二维质子磁共振光谱、红外光谱、负离子质谱、溶剂解以及薄层色谱上的免疫染色，推测该糖脂的结构为HSO3-3Glcβ1-1Cer（其中Cer为神经酰胺）。神经酰胺部分由4-D-羟基鞘氨醇作为唯一的长链碱基组成，脂肪酸主要由二十四烷酸组成，这是通过组成分析和负离子质谱推断得出的。葡萄糖硫酸脑苷脂的产量约为5 nmol/g组织，约为乳糖基硫酸神经酰胺产量的三倍。
