Tadano K, Ishizuka I
J Biol Chem. 1982 Aug 25;257(16):9294-9.
A novel sulfoglycosphingolipid containing two sulfate ester groups was isolated from the lipid extract of rat kidney. The isolation procedure involved extraction of lipids with chloroform/methanol, alkaline methanolysis, and column chromatographies with DEAE-Sephacel and silica beads. By infrared spectroscopy, proton magnetic resonance spectroscopy, periodate oxidation, solvolysis, chromium trioxide oxidation, and methylation analysis of the native and partially degraded compounds, the structure of this glycolipid is proposed to be (HSO3-3)GalNAc beta 1-4(HSO3-3)Gal beta 1-4Glc beta 1-1Cer. The presence of a unique 3-O-sulfated N-acetylgalactosamine structure was confirmed by gas chromatography-mass spectrometry. The yield of this sulfoglycolipid was 11.2 nmol/g of tissue, which was about half of that of monosulfogangliotriaosylceramide from rat kidney.
从大鼠肾脏的脂质提取物中分离出一种含有两个硫酸酯基团的新型硫代糖鞘脂。分离过程包括用氯仿/甲醇提取脂质、碱性甲醇解以及使用DEAE-葡聚糖凝胶和硅胶珠进行柱色谱分离。通过对天然和部分降解化合物进行红外光谱、质子磁共振光谱、高碘酸盐氧化、溶剂解、三氧化铬氧化和甲基化分析，推测这种糖脂的结构为(HSO3-3)GalNAcβ1-4(HSO3-3)Galβ1-4Glcβ1-1Cer。通过气相色谱-质谱法证实了独特的3-O-硫酸化N-乙酰半乳糖胺结构的存在。这种硫代糖脂的产量为11.2 nmol/g组织，约为大鼠肾脏中单硫酸神经节三糖神经酰胺产量的一半。
