Tadano K, Ishizuka I
J Biol Chem. 1982 Feb 10;257(3):1482-90.
A sulfoglycosphingolipid containing N-acetylgalactosamine has been isolated from the lipid extract of rat kidney. The isolation procedure involved extraction of lipids with chloroform/methanol, alkaline methanolysis, and column chromatographies with DEAE-Sephadex and Silica beads. The components were N-acetylgalactosamine, galactose, glucose, sphingoid bases, fatty acids, and sulfate in equimolar amounts. The yield of this sulfoglycolipid was about 24 nmol/g of tissue, which was about 13% of that of galactosylceramide 3-sulfate from rat kidney. By infrared spectroscopy, proton magnetic resonance spectroscopy, periodate oxidation, solvolysis, chromium trioxide oxidation, and methylation analysis of the native and partially degraded compound, the structure of this glycolipid is proposed to be GalNAc beta 1--4(HSO3-3)Gal beta 1--4Glc beta 1--1Cer.
一种含有N-乙酰半乳糖胺的硫糖鞘脂已从大鼠肾脏的脂质提取物中分离出来。分离过程包括用氯仿/甲醇提取脂质、碱性甲醇解以及用DEAE-葡聚糖和硅胶珠进行柱色谱分离。其成分包括等摩尔量的N-乙酰半乳糖胺、半乳糖、葡萄糖、鞘氨醇碱、脂肪酸和硫酸盐。这种硫糖脂的产量约为24 nmol/g组织，约为大鼠肾脏中硫酸半乳糖基神经酰胺产量的13%。通过对天然和部分降解化合物的红外光谱、质子磁共振光谱、高碘酸盐氧化、溶剂解、三氧化铬氧化和甲基化分析，推测这种糖脂的结构为GalNAcβ1--4(HSO3-3)Galβ1--4Glcβ1--1Cer。
