Tadano K, Ishizuka I, Matsuo M, Matsumoto S
J Biol Chem. 1982 Nov 25;257(22):13413-20.
A novel bis-sulfated tetraglycosylceramide containing N-acetylgalactosamine was isolated from the lipid extract of rat kidney. The isolation procedure involved extraction of lipids with chloroform/methanol, alkaline methanolysis, and column chromatographies with DEAE-Sephacel and silica beads. By infrared spectroscopy, proton magnetic resonance spectroscopy, periodate oxidation, solvolysis, chromium trioxide oxidation, and methylation analysis of the native and partially degraded compounds, the structure of this glycolipid is proposed to be (HSO3-3)Gal beta 1-3GalNAc beta 1-4(HSO3-3)Gal beta 1-4Glc beta 1-1Cer. The position of sulfate ester groups and the sequence of carbohydrates were further confirmed by methylation analysis and direct probe mass spectrometry after desulfation-trideuteriomethylation of permethylated glycolipids. The yield of this sulfoglycolipid was 5.5 nmol/g of tissue, which was about 25 and 50 mol %, respectively, of monosulfo-and bis-sulfogangliotriaosylceramide from rat kidney.
从大鼠肾脏的脂质提取物中分离出一种含有N-乙酰半乳糖胺的新型双硫酸化四糖基神经酰胺。分离过程包括用氯仿/甲醇提取脂质、碱性甲醇解以及使用DEAE-葡聚糖凝胶和硅胶珠进行柱色谱分离。通过红外光谱、质子磁共振光谱、高碘酸盐氧化、溶剂解、三氧化铬氧化以及对天然和部分降解化合物的甲基化分析，推测这种糖脂的结构为(HSO3-3)Galβ1-3GalNAcβ1-4(HSO3-3)Galβ1-4Glcβ1-1Cer。在对全甲基化糖脂进行脱硫-三氘代甲基化后，通过甲基化分析和直接探针质谱进一步证实了硫酸酯基团的位置和碳水化合物的序列。这种硫糖脂的产量为5.5 nmol/g组织，分别约为大鼠肾脏中单硫酸化和双硫酸化神经节三糖基神经酰胺的25%和50%。
