Giri Praveen K, Lu Yang, Lei Shan, Li Weisong, Zheng Juan, Lu Haixia, Chen Xinlin, Liu Yong, Zhang Pengbo
Department of Anesthesiology, The Second Affiliated Hospital of Xi'an Jiaotong University.
Department of Anesthesiology and Critical Care, Teaching Hospital of Karnali Academy of Health Sciences, Jumla, Nepal.
Neuroreport. 2018 Feb 7;29(3):153-159. doi: 10.1097/WNR.0000000000000937.
Laboratory studies suggested that general anesthetics induce neuroapoptosis and inhibit neurogenesis in developing brains of animals. Minocycline exerts neuroprotection against a wide range of toxic insults in neurodegenerative diseases models. Here, we investigate whether minocycline can alleviate neurogenetic damage and improve cognition following midazolam exposure in neonatal rats. Postnatal 7 days rats were divided randomly into three groups: control group (C), midazolam group (M), and minocycline pretreatment group (MP). After exposure to midazolam, the cell proliferation in the subventricular zone (SVZ) and the subgranular zone (SGZ) of the hippocampus in pups was analyzed by bromodeoxyuridine immunochemistry at 7 days after the administration of anesthesia. Cognitive function was assessed using the Morris water-maze test at 35 days after midazolam exposure. Compared with the control, midazolam reduced cell proliferation both in the SVZ and in the SGZ of the hippocampus of neonatal rats, and decreased spatial learning and memory ability of rats in adulthood significantly. Pretreatment with minocycline increased cell proliferation both in the SVZ and in the SGZ of the hippocampus and improved spatial learning and memory ability compared with midazolam, but it did not mitigate the changes to the normal levels compared with the controls. Our results indicated that pretreatment with minocycline can alleviate midazolam-induced damage in neural stem cell proliferation of neonatal rats and improve spatial learning and memory ability of rats in adulthood.
实验室研究表明，全身麻醉剂可诱导动物发育中的大脑发生神经细胞凋亡并抑制神经发生。米诺环素在神经退行性疾病模型中对多种毒性损伤具有神经保护作用。在此，我们研究米诺环素是否能减轻新生大鼠在咪达唑仑暴露后的神经发生损伤并改善认知功能。将出生后7天的大鼠随机分为三组：对照组（C）、咪达唑仑组（M）和米诺环素预处理组（MP）。在暴露于咪达唑仑后，于麻醉给药后7天通过溴脱氧尿苷免疫化学分析幼崽海马体室下区（SVZ）和颗粒下区（SGZ）的细胞增殖情况。在咪达唑仑暴露后35天使用莫里斯水迷宫试验评估认知功能。与对照组相比，咪达唑仑降低了新生大鼠海马体SVZ和SGZ中的细胞增殖，并显著降低了成年大鼠的空间学习和记忆能力。与咪达唑仑相比，米诺环素预处理增加了海马体SVZ和SGZ中的细胞增殖并改善了空间学习和记忆能力，但与对照组相比，它并未将这些变化减轻至正常水平。我们的结果表明，米诺环素预处理可减轻咪达唑仑诱导的新生大鼠神经干细胞增殖损伤，并改善成年大鼠的空间学习和记忆能力。
