Allam Gamal, Nasr Amre, Talaat Iman M, Abuelsaad Abdelaziz S A, Bakheit Ali M, Nemenqani Dalal, Alsulaimani Adnan A
a Department of Microbiology and Immunology , College of Medicine, Taif University , Taif , Saudi Arabia.
b Immunology Section, Department of Zoology, Faculty of Science , Beni-Suef University , Beni-Suef , Egypt.
Immunol Invest. 2018 Apr;47(3):229-240. doi: 10.1080/08820139.2017.1416398. Epub 2017 Dec 19.
Association studies of genes encoding cytokines that play an important role in inflammatory response represent one approach to finding type 1 diabetes (T1D) disease genes. The aim of this study was to investigate the association of single nucleotide polymorphisms (SNPs) within cytokine genes with T1D in a cohort of Saudi subjects.
A total of 300 well-characterized type 1 diabetic patients and 300 T1D-free control subjects were enrolled in this investigation. Cytokine SNPs were genotyped by using Polymerase chain reaction (PCR) with sequence-specific primers.
Our data revealed that IFN-γ +874T allele carriers [odds ratio (OR) = 1.87, p < 0.001] and TT homozygotes (OR = 1.28, p < 0.001) were significantly more susceptible to developing T1D than the A allele carriers. In addition, TNF-α -308A allele carriers (OR = 1.73, p < 0.001) and AA homozygotes (OR = 1.74, p < 0.001) were also overrepresented among the diabetics than G allele carriers. IL-4 -590C/T TT homozygotes (OR = 2.23, p < 0.001) were significantly more susceptible to develop T1D than CC genotypes, whereas CT heterozygotes were not significantly associated (OR = 1.43, p = 0.78) with T1D. Furthermore, IL-4 T allele was statistically associated with T1D patients compared to control group (OR = 2.24, p < 0.001). Similarly, IL-1β -511C/T TT homozygotes (OR = 1.85, p = 0.012) and the T allele (OR = 1.85, p < 0.001) were significantly more susceptible to T1D than CC genotypes, whereas TC heterozygotes (OR = 1.04, p = 0.86) were not significantly associated with T1D.
Our data concluded that IFN-γ +874T allele, TNF-α -308A allele, IL-1β -511T allele, and IL-4 -590T allele could be considered risk factors for T1D development in Saudi subjects.
对在炎症反应中起重要作用的细胞因子编码基因进行关联研究是寻找1型糖尿病（T1D）疾病基因的一种方法。本研究的目的是在一组沙特受试者中调查细胞因子基因内单核苷酸多态性（SNP）与T1D的关联。
本研究共纳入300例特征明确的1型糖尿病患者和300例无T1D的对照受试者。采用聚合酶链反应（PCR）和序列特异性引物对细胞因子SNP进行基因分型。
我们的数据显示，与A等位基因携带者相比，IFN-γ +874T等位基因携带者[优势比（OR）=1.87，p<0.001]和TT纯合子（OR=1.28，p<0.001）患T1D的易感性显著更高。此外，与G等位基因携带者相比，TNF-α -308A等位基因携带者（OR=1.73，p<0.001）和AA纯合子（OR=1.74，p<0.001）在糖尿病患者中的比例也更高。IL-4 -590C/T TT纯合子（OR=2.23，p<0.001）患T1D的易感性显著高于CC基因型，而CT杂合子与T1D无显著关联（OR=1.43，p=0.78）。此外，与对照组相比，IL-4 T等位基因与T1D患者在统计学上相关（OR=2.24，p<0.001）。同样，与CC基因型相比，IL-1β -511C/T TT纯合子（OR=1.85，p=0.012）和T等位基因（OR=1.85，p<0.001）患T1D的易感性显著更高，而TC杂合子与T1D无显著关联（OR=1.04，p=0.86）。
我们的数据得出结论，IFN-γ +874T等位基因/TNF-α -308A等位基因、IL-1β -511T等位基因和IL-4 -590T等位基因可被视为沙特受试者发生T1D的危险因素。
