Zhen Shiyu, Chen Si, Geng Sheng, Zhang Hao, Chen Yongsheng, Liu Benguo
School of Food Science, Henan Institute of Science and Technology, Xinxiang 453003, China.
Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China.
Foods. 2022 Feb 24;11(5):668. doi: 10.3390/foods11050668.
We performed ultrasound-assisted extraction coupled with natural deep eutectic solvents (NADES) to achieve the green and efficient preparation of flavonoid extract from leaves. We then evaluated its antioxidant and antiproliferative activities. A NADES consisting of choline chloride and glucose at a molar ratio of 4:1 with 20% water was determined to be the most suitable solvent. The optimal extraction conditions were: a liquid-to-solid ratio of 30 mL/g, an ultrasonication power of 490 W, and an ultrasonication time of 6.5 min. The actual flavonoid yield was 83.93%, which was close to the predicted yield. Further, 86.75% of the flavonoids were recovered by adding the same volume of phosphate buffer saline (100 mM, pH of 7.0) to the extract solution. Although the chemical antioxidant activities of the flavonoid extract were slightly inferior to those of dihydromyricetin, the flavonoid extract could still effectively inhibit the proliferation of human breast MDA-MB-231 cells by inducing cell apoptosis, retarding the cell cycle, changing the mitochondrial membrane potential and scavenging intracellular reactive oxygen species (ROS). The obtained results can provide a reference in the development of plant-derived functional foods.
我们采用超声辅助提取结合天然深共熔溶剂（NADES）的方法，实现了从叶片中绿色高效地制备黄酮提取物。然后，我们评估了其抗氧化和抗增殖活性。确定了一种由摩尔比为4:1的氯化胆碱和葡萄糖与20%水组成的NADES为最合适的溶剂。最佳提取条件为：液固比30 mL/g、超声功率490 W、超声时间6.5 min。实际黄酮产率为83.93%，接近预测产率。此外，通过向提取液中加入相同体积的磷酸盐缓冲盐水（100 mM，pH值7.0），回收了86.75%的黄酮。虽然黄酮提取物的化学抗氧化活性略低于二氢杨梅素，但该黄酮提取物仍可通过诱导细胞凋亡、阻滞细胞周期、改变线粒体膜电位和清除细胞内活性氧（ROS），有效抑制人乳腺癌MDA-MB-231细胞的增殖。所得结果可为植物源功能性食品的开发提供参考。
