Department of Chemistry, Mahabad Branch, Islamic Azad University, Mahabad, Islamic Republic of Iran.
Research Center for Health, Safety and Environment (RCHSE), Alborz University of Medical Sciences, Karaj, Iran.
Environ Sci Pollut Res Int. 2018 Mar;25(7):6739-6750. doi: 10.1007/s11356-017-0974-2. Epub 2017 Dec 20.
The effect of nanotitanium dioxide (TiO-NP) in human monocytes is still unknown. Therefore, an understanding of probable cytotoxicity of TiO-NP on human monocytes and underlining the mechanisms involved is of significant interest. The aim of this study was to assess the cytotoxicity of TiO-NP on human monocytes. Using biochemical and flow cytometry assessments, we demonstrated that addition of TiO-NP at 10 μg/ml concentration to monocytes induced cytotoxicity following 12 h. The TiO-NP-induced cytotoxicity on monocytes was associated with intracellular reactive oxygen species (ROS) generation, mitochondrial membrane potential (MMP) collapse, lysosomal membrane injury, lipid peroxidation, and depletion of glutathione. According to our results, TiO-NP triggers oxidative stress and organelles damages in monocytes which are important cells in defense against foreign agents. Finally, our findings suggest that use of antioxidants and mitochondrial/lysosomal protective agents could be of benefit for the people in the exposure with TiO-NP.
纳米二氧化钛（TiO-NP）对人单核细胞的影响尚不清楚。因此，了解 TiO-NP 对人单核细胞可能的细胞毒性及其相关机制具有重要意义。本研究旨在评估 TiO-NP 对人单核细胞的细胞毒性。通过生化和流式细胞术评估，我们证明了将浓度为 10μg/ml 的 TiO-NP 添加到人单核细胞中 12 小时后会诱导细胞毒性。TiO-NP 诱导的单核细胞毒性与细胞内活性氧（ROS）生成、线粒体膜电位（MMP）崩溃、溶酶体膜损伤、脂质过氧化和谷胱甘肽耗竭有关。根据我们的结果，TiO-NP 会引发单核细胞中的氧化应激和细胞器损伤，单核细胞是抵御外来物质的重要细胞。最后，我们的研究结果表明，使用抗氧化剂和线粒体/溶酶体保护剂可能对暴露于 TiO-NP 的人群有益。
