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二氧化钛和氧化锌纳米颗粒的细胞毒性及DNA损伤评估。在培养的肺细胞中的摄取情况。

Cytotoxicity and DNA damage evaluation of TiO and ZnO nanoparticles. Uptake in lung cells in culture.

作者信息

Freire K, Ordóñez Ramos F, Soria D B, Pabón Gelves E, Di Virgilio A L

机构信息

CEQUINOR, (CONICET-UNLP), Bv. 120 N 1465, La Plata, Argentina.

Escuela de Química, Facultad de Ciencias, Universidad Nacional de Colombia, sede Medellín. Cra 65 #59A -110, Medellín, Colombia.

出版信息

Toxicol Res (Camb). 2021 Mar 9;10(2):192-202. doi: 10.1093/toxres/tfaa112. eCollection 2021 Mar.

DOI:10.1093/toxres/tfaa112
PMID:33884170
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8045598/
Abstract

The cytotoxicity and DNA damage of titanium dioxide and zinc oxide nanoparticles (TiO and ZnO NPs) have been studied in a human lung carcinoma cell line (A549) after 24 h exposure. TiO and ZnO NPs had mean diameters of 12.9 ± 2.8 and 24.1 ± 8.0 nm, respectively. ZnO NPs reduced cell viability from 250 μg/mL, increasing reactive oxygen species (ROS) and decreased GSH/GSSG ratio. The comet assay detected DNA damage from 50 μg/mL. TiO NPs induced cytotoxicity and DNA damage from 50 to 100 μg/mL, respectively, along with a decrease of the GSH/GSSG ratio. Both particles were found inside the cells, within membrane-bound vesicles. The internalization mechanism is promoted partially by caveolae-mediated endocytosis and, in the case of TiO NPs, also by macropinocytosis.

摘要

在暴露24小时后，研究了二氧化钛和氧化锌纳米颗粒（TiO和ZnO NPs）对人肺癌细胞系（A549）的细胞毒性和DNA损伤。TiO和ZnO NPs的平均直径分别为12.9±2.8和24.1±8.0纳米。ZnO NPs在250μg/mL时降低细胞活力，增加活性氧（ROS）并降低GSH/GSSG比值。彗星试验检测到50μg/mL时的DNA损伤。TiO NPs分别在50至100μg/mL时诱导细胞毒性和DNA损伤，同时GSH/GSSG比值降低。两种颗粒都存在于细胞内的膜结合囊泡中。内化机制部分由小窝介导的内吞作用促进，对于TiO NPs，还由巨胞饮作用促进。

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