多种结构揭示了 Sec 蛋白的秘密。
Multiple Structures Disclose the Secretins' Secrets.
机构信息
Imperial College London, Department of Life Sciences, MRC-CMBI, London, United Kingdom
Aix Marseille University, CNRS, IMM, LISM, Marseille, France.
出版信息
J Bacteriol. 2018 Feb 7;200(5). doi: 10.1128/JB.00702-17. Print 2018 Mar 1.
Abstract
Bacterial secretins are outer membrane proteins that provide a path for secreted proteins to access the cell exterior/surface. They are one of the core components of secretion machines and are found in type II and type III secretion systems (T2SS and T3SS, respectively). The secretins comprise giant ring-shaped homo-oligomers whose precise atomic organization was only recently deciphered thanks to spectacular developments in cryo-electron microscopy (cryo-EM) imaging techniques.
摘要
细菌分泌蛋白是一种外膜蛋白，为分泌蛋白进入细胞外表面/表面提供途径。它们是分泌机器的核心组件之一，存在于 II 型和 III 型分泌系统（分别为 T2SS 和 T3SS）中。分泌蛋白由巨大的环形同源寡聚体组成，其精确的原子结构最近才由于冷冻电子显微镜（cryo-EM）成像技术的显著发展而被破解。
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3
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