检测G蛋白偶联受体CXCR4的细胞表面表达
Detecting Cell Surface Expression of the G Protein-Coupled Receptor CXCR4.
作者信息
Nevins Amanda M, Marchese Adriano
机构信息
Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI, USA.
出版信息
Methods Mol Biol. 2018;1722:151-164. doi: 10.1007/978-1-4939-7553-2_10.
Abstract
G protein-coupled receptors (GPCRs) are cell surface receptors that relay extracellular signals to the inside of the cells. C-X-C chemokine receptor 4 (CXCR4) is a GPCR that undergoes receptor internalization and recycling upon stimulation with its cognate ligand, C-X-C chemokine 12 (CXCL12). Using this receptor/ligand pair we describe the use of two techniques, enzyme-linked immunosorbent assay (ELISA) and flow cytometry, widely used to quantify GPCR internalization from the plasma membrane and its return to the cell surface by recycling.
摘要
G蛋白偶联受体（GPCRs）是细胞表面受体，可将细胞外信号传递至细胞内部。C-X-C趋化因子受体4（CXCR4）是一种GPCR，在受到其同源配体C-X-C趋化因子12（CXCL12）刺激后会发生受体内化和再循环。利用这一受体/配体对，我们描述了两种技术的应用，即酶联免疫吸附测定（ELISA）和流式细胞术，这两种技术广泛用于量化质膜上GPCR的内化及其通过再循环返回细胞表面的过程。
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