Lima Gláucia Moreira Espíndola, Nunes Maína de Oliveira, Chang Marilene Rodrigues, Tsujisaki Rosianne Assis de Sousa, Nunes Joslaine de Oliveira, Taira Cleison Ledesma, Thomaz Danilo Yamamoto, Negro Gilda Maria Bárbaro Del, Mendes Rinaldo Pôncio, Paniago Anamaria Mello Miranda
Universidade Federal de Mato Grosso do Sul, Hospital Universitário Maria Aparecida Pedrossian, Laboratório de Análises Clínicas, Campo Grande, Mato Grosso do Sul, Brazil.
Universidade Federal de Mato Grosso do Sul, Faculdade de Ciências Farmacêuticas, Alimentos e Nutrição, Campo Grande, Mato Grosso do Sul, Brazil.
Rev Inst Med Trop Sao Paulo. 2017 Dec 21;59:e75. doi: 10.1590/S1678-9946201759075.
The aim of this study was to identify Candida spp. isolated from candiduria episodes at a tertiary hospital in the Midwest region of Brazil, and to determine their susceptibility profiles to antifungal compounds. From May 2011 to April 2012, Candida spp. isolated from 106 adult patients with candiduria admitted to the University Hospital of the Federal University of Mato Grosso do Sul were evaluated. Both, species identification and susceptibility testing with fluconazole-FLC, voriconazole-VRC, and amphotericin B-AmB were carried out using the Vitek 2. To discriminate species of the C. parapsilosis complex, a RAPD-PCR technique using the RPO2 primer was performed. From the total of 106 isolates, 42 (39.6%) C. albicans and 64 (60.4%) Candida non-albicans (CNA) - 33 C. tropicalis, 18 C. glabrata, 5 C. krusei, 4 C. parapsilosis sensu stricto, 2 C. kefyr, 1 C. lusitaniae, and 1 C. guilliermondii were identified. All isolates were susceptible to AmB and VRC, whereas all C. glabrata isolates presented either resistance (5.6%) or dose-dependent susceptibility (94.4%) to FLC. The study of Candida spp. and their resistance profiles may help in tailoring more efficient therapeutic strategies for candiduria.
本研究的目的是鉴定从巴西中西部地区一家三级医院的念珠菌尿病例中分离出的念珠菌属，并确定它们对抗真菌化合物的敏感性谱。2011年5月至2012年4月，对从南马托格罗索联邦大学大学医院收治的106例成年念珠菌尿患者中分离出的念珠菌属进行了评估。使用Vitek 2进行念珠菌属鉴定以及对氟康唑（FLC）、伏立康唑（VRC）和两性霉素B（AmB）的敏感性测试。为了区分近平滑念珠菌复合体的菌种，使用RPO2引物进行了RAPD-PCR技术检测。在总共106株分离株中，鉴定出42株（39.6%）白色念珠菌和64株（60.4%）非白色念珠菌（CNA）——33株热带念珠菌、18株光滑念珠菌、5株克柔念珠菌、4株狭义近平滑念珠菌、2株解脂念珠菌、1株葡萄牙念珠菌和1株季也蒙念珠菌。所有分离株对AmB和VRC敏感，而所有光滑念珠菌分离株对FLC呈现耐药（5.6%）或剂量依赖性敏感（94.4%）。对念珠菌属及其耐药谱的研究可能有助于为念珠菌尿制定更有效的治疗策略。
